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Pulmonary alveolar proteinosis: A spectrum of cytologic, histochemical, and ultrastructural findings in bronchoalveolar lavage fluid
Maygarden, SJ; Iacocca, MV; Funkhouser, WK; Novotny, DB;
Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 b Med, Chapel Hill, NC 27599 USA
Titolo Testata:
fascicolo: 6, volume: 24, anno: 2001,
pagine: 389 - 395
cytology; bronchoalveolar lavage; pulmonary alveolar proteinosis;
Tipo documento:
Settore Disciplinare:
Clinical Medicine
Indirizzi per estratti:
Indirizzo: Maygarden, SJ Univ N Carolina, Dept Pathol & Lab Med, CB 7525, Chapel Hill, NC 27599 USA Univ N Carolina CB 7525 Chapel Hill NC USA 27599 C 27599 USA
S.J. Maygarden et al., "Pulmonary alveolar proteinosis: A spectrum of cytologic, histochemical, and ultrastructural findings in bronchoalveolar lavage fluid", DIAGN CYTOP, 24(6), 2001, pp. 389-395


Pulmonary alveolar proteinosis (PAP) is defined as abundant extracellular proteinaceous periodic acid-Schiff (PAS)-positive material which representssurfactant distending alveolar spaces. While this lesion is defined by histologic findings, there are characteristic radiologic features and cytologic findings in bronchoalveolar lavage (BAL) specimens that together may provide a confident diagnosis. The BAL specimens fro,ll all patients for which a diagnosis of PAP was made or suggested on either cytologic or biopsy specimens at University of North Carolina Hospitals from 1990-1999,were reviewed. There were 23 cytologicspecimens from ii patients. Patient ages ranged from 6 wk to 76 yr: All 23specimens had slides prepared for Papanicolaou stain, 22 specimens (all patients) had Diff-Quik stains, 10 specimens (6 patients) had PAS stains, and8 specimens (5 patients) had lipid stains. Nine patients had lung biopsiesin addition to cytologic specimens. The clinical charts of all patients were reviewed. Twenty-one cytologic specimens were described as cloudy or milky and 2 were bloody. By chart review and/or biopsy results, 8 patients were felt to have definite PAP. The initial lavage specimens fr om 6 of these patients showed classic cytologic findings of PAP, consisting of paucicellular specimens dominated by adundant extracellular granular to globular material which was basophilic on Diff-Quik stain, pale to focally eosinophilic on Pap stain, and PAS-positive, diastase-resistant. Five of these patients had biopsies; 3 showed PAP, and were insufficient. Later BAL specimens after therapeutic lavagefrom these patients were often less characteristic, with scant extracellular material present. The other 2 patients with PAP clinically and bybiopsy had atypical cytologic findings, with one showing numerous macrophages with scant PAS-positive material and abundant lipid mimicking lipid pneumonia, and one showing moderate eosinophils in addition to the extracellular proteinacous material. The remaining 3 patients were felt not to have PAP clinically or by biopsy (1 lymphocytic interstitial pneumonitis, 1 rheumatoid lung, and 1 hemosiderosis), and their BAL specimens predominantly contained macrophages with are proteinaceous extracellular globules. Electron microscopy was performed in 5 patients (4 considered to have PAP, and I withlymphocytic interstitial pneumonitis) and in all cases showed whorled myelin figures characteristic of surfactant. The PAP cases and the non-PAP casehad identical ultrastructural findings. We conclude that BAL specimens with classic cytologic features and supporting clinical and radiographic evidence may be diagnosed as PAP. Atypical specimens should be approached,vith caution, and may represent either PAP or other pulmonary diseases with secondary accumulation of surfactant. Cytology specimens taken subsequent to therapeutic lavage from PAP patients may also not be diagnostic. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 05:43:54