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Titolo:
A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of gene expression during muscle development
Autore:
Carvajal, JJ; Cox, D; Summerbell, D; Rigby, PWJ;
Indirizzi:
Natl Inst Med Res, Div Eukaryot Mol Genet, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA Genet, London NW7 1AA, England
Titolo Testata:
DEVELOPMENT
fascicolo: 10, volume: 128, anno: 2001,
pagine: 1857 - 1868
SICI:
0950-1991(200105)128:10<1857:ABTAOT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRANIAL PARAXIAL MESODERM; SKELETAL-MUSCLE; MOUSE EMBRYO; ARTIFICIAL CHROMOSOMES; REGULATORY GENE; DISTINCT; MYF-5; MYOD; MYOGENIN; MEMBER;
Keywords:
Myf5; Mrf4; MRF myogenesis; muscle; branchial arch; epaxial; hypaxial; hyoid; mandibular; craniofacial; BAC; transcriptional regulation; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Rigby, PWJ Inst Canc Res, Sect Gene Funct & Regulat, Chester Beatty Labs, 237 Fulham Rd, London SW3 6JB, England Inst Canc Res 237 Fulham Rd London England SW3 6JB JB, England
Citazione:
J.J. Carvajal et al., "A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of gene expression during muscle development", DEVELOPMENT, 128(10), 2001, pp. 1857-1868

Abstract

The muscle-specific transcription factors Myf5 and Mrf4 are two of the four myogenic regulatory factors involved in the transcriptional cascade responsible for skeletal myogenesis in the vertebrate embryo. Myf5 is the first of these four genes to be expressed in the mouse, We have previously described discrete enhancers that drive Myf5 expression in epaxial and hypaxial somites, branchial arches and central nervous system, and argued that additional elements are required for proper expression (Summerbell, D., Ashby, P. R., Coutelle, O., Cox, D., Yee, S, P, and Rigby, P, W J, (2000) Development 127, 3745-3757). We have now investigated the transcriptional regulation of both MyfS and Mrf4 using bacterial artificial chromosome transgenesis, We show that a clone containing MyfS and 140 kb of upstream sequences is sufficient to recapitulate the known expression patterns of both genes. Our results confirm and reinforce the conclusion of our earlier studies, that MyfS expression is regulated differently in each of a considerable number of populations of muscle progenitors, and they begin to illuminate the evolutionary origins of this complex regulation, We further show that separate elements are involved in the activation and maintenance of expression in the various precursor populations, reflecting the diversity of the signals that control myogenesis, Mrf4 expression requires at least four elements, one of which may be shared with Myf5, providing a possible explanation for the linkage of these genes throughout vertebrate phylogeny, Further complexity is revealed by the demonstration that elements which control Mrf4 and Myf5 areembedded in an unrelated neighbouring gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:21:03