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Titolo:
Sequential measurement of human herpesvirus 6 DNA with polymerase chain reaction method in pediatric living-related liver transplantation
Autore:
Hasegawa, T; Kimura, T; Sasaki, T; Okada, A; Tanaka-Taya, K; Miyagawa, H; Amou, K; Mushiake, S; Yamanishi, K; Ishikawa, S;
Indirizzi:
Osaka Univ, Sch Med, Dept Pediat Surg, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 at Surg, Suita, Osaka 5650871, Japan
Titolo Testata:
CLINICAL TRANSPLANTATION
fascicolo: 3, volume: 15, anno: 2001,
pagine: 208 - 213
SICI:
0902-0063(200106)15:3<208:SMOHH6>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDIOPATHIC THROMBOCYTOPENIC PURPURA; RENAL-TRANSPLANTATION; HUMAN-HERPESVIRUS-6 INFECTION; PERIPHERAL-BLOOD; EXANTHEM-SUBITUM; CYTOMEGALOVIRUS; RECIPIENTS; CHILDREN; IDENTIFICATION; DISEASE;
Keywords:
exanthem subitum; human herpesvirus 6; pediatric living-related liver transplantation; polymerase chain reaction; tacrolimus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Hasegawa, T Osaka Univ, Sch Med, Dept Pediat Surg, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
T. Hasegawa et al., "Sequential measurement of human herpesvirus 6 DNA with polymerase chain reaction method in pediatric living-related liver transplantation", CLIN TRANSP, 15(3), 2001, pp. 208-213

Abstract

Background: Human herpesvirus 6 (HHV-6), a causative virus of exanthem subitum, may occasionally present with a severe clinical form in immunosuppressed patients after transplantation. In this study, HHV-6 DNA was sequentially measured with a polymerase chain reaction (PCR) method, a quick and sensitive modality in pediatric living-related liver transplantation (LTx). Methods: Subjects consisted of 5 post-operative biliary atresia patients undergoing living-related LTx at ages from 8 months to 4 yr. Immunosuppression was performed with Tacrolimus (blood trough level 8-18 within 1 month and 5-10 ng/mL thereafter) and low-dose steroid. Specimens were peripheral blood mononuclear cells (PBMC), plasma, and liver biopsy tissue. The amount of HHV-6 DNA was semiquantified as follows: If, 1-10; 2+, 10-100; 3+, 100-1000; 4+, over 1000 copies/10(5) PBMCs. Results: A total of 69 blood samples and three liver biopsies were provided for the examination. HHV-6 DNA in PBMC was positive in 2 donors and 3 recipients before LTx. Two patients with negative DNA were converted to 3 + at2-3 wk after LTx and 3 with positive DNA remained 2+ to 3 + throughout thepost-LTx period. Only 1 patient developed clinical symptoms, such as fever, liver dysfunction, petechiae, idiopathic thrombocytopenic purpura, and finally bone marrow suppression. HHV-6 DNA in the liver biopsy tissue and plasma in this patient were 4+ and 2+, respectively. Conclusion: HHV-6 DNA in PBMC measured by the PCR method may be persistently high in pediatric recipients after living-related LTx. Although HHV-6 DNA in PBMC may be positive in case of evident infection, positivity in PBMC may not be always associated with the clinical symptoms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:49:21