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Titolo:
Detection of differentially expressed genes in HeLa x fibroblast hybrids using subtractive suppression hybridization
Autore:
Nishizuka, SS; Tsujimoto, HY; Stanbridge, EJ;
Indirizzi:
Univ Calif Irvine, Coll Med, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 l & Mol Genet, Irvine, CA 92697 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 11, volume: 61, anno: 2001,
pagine: 4536 - 4540
SICI:
0008-5472(20010601)61:11<4536:DODEGI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CELL HYBRIDS; CYCLE ARREST; IDENTIFICATION; TUMORIGENICITY; CDNA; TRANSFORMATION; CHROMOSOME-11; CARCINOMA; DELETION; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Nishizuka, SS NCI, Mol Pharmacol Lab, NIH, Bldg 37,Room 4C-09,9000 Rockville Pike, Bethesda, MD 20892 USA NCI Bldg 37,Room 4C-09,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
S.S. Nishizuka et al., "Detection of differentially expressed genes in HeLa x fibroblast hybrids using subtractive suppression hybridization", CANCER RES, 61(11), 2001, pp. 4536-4540

Abstract

To understand genetic differences and similarities between tumorigenic andnontumorigenic HeLa x fibroblast hybrid cells, subtractive suppression hybridization (SSH), based on suppression PCR and a combination of normalization and subtraction in a single procedure, was used. Using the nontumorigenic CGL1 and tumorigenic CGL3, forward (CGL1-CGL3) and reverse (CGL3-CGL1) subtracted libraries mere constructed. Among 192 clones, seven were identified as differentially expressed genes specific for either CGL1 or CGL3. All seven were not reported previously as differentially expressed genes in thishybrid system, In the forward subtraction, p16 was isolated, indicating the involvement of the loss of tumorigenic phenotype, Subsequent transfectionof wild-type p16 to the tumorigenic CGL3 showed growth suppression in colony formation assay; however, no tumor suppression was observed when the transfectant was inoculated into nude mice. These results indicate that: (a) SSH is a suitable method to identify differentially expressed genes in two types of cells; and (b) although p16 plays some roles in growth suppression,the p16-transfected CGL3 is still capable to proliferate in vivo.

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Documento generato il 20/09/20 alle ore 05:46:24