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Titolo:
Involvement of the NH2 terminal domain of catecholamine transporters in the Na+ and Cl--dependence of a [H-3]-dopamine uptake
Autore:
Syringas, M; Janin, F; Giros, B; Costentin, J; Bonnet, JJ;
Indirizzi:
UFR Med & Pharm, UMR CNRS 6036, IFRMP 23, F-76000 Rouen, France UFR Med & Pharm Rouen France F-76000 36, IFRMP 23, F-76000 Rouen, France INSERM U513, F-94000 Creteil, France INSERM U513 Creteil France F-94000INSERM U513, F-94000 Creteil, France
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 133, anno: 2001,
pagine: 387 - 394
SICI:
0007-1188(200106)133:3<387:IOTNTD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOPAMINE-NOREPINEPHRINE TRANSPORTERS; STRUCTURAL DOMAINS; NEURONAL UPTAKE; CHLORIDE DEPENDENCE; KINETIC-ANALYSIS; ION DEPENDENCE; GBR 12783; COCAINE; NORADRENALINE; EXPRESSION;
Keywords:
human dopamine transporter; human noradrenaline transporter; chimeric transporters; ions; dopamine transport; Ltk(-) transfected cells; COS-7 transfected cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Bonnet, JJ UFR Med & Pharm, UMR CNRS 6036, IFRMP 23, 22 Blvd Gambetta, F-76000 Rouen,France UFR Med & Pharm 22 Blvd Gambetta Rouen France F-76000 n,France
Citazione:
M. Syringas et al., "Involvement of the NH2 terminal domain of catecholamine transporters in the Na+ and Cl--dependence of a [H-3]-dopamine uptake", BR J PHARM, 133(3), 2001, pp. 387-394

Abstract

1 The ionic dependence of the [H-3]-dopamine uptake was studied in transfected cells expressing the human neuronal transporter fur dopamine (hDAT) ornoradrenaline (hNET), and chimeric transporters resulting from the symmetrical exchange of the region from the NH2 terminal through the first two transmembrane domains (cassette I). Chimera A is formed by hDAT comprising cassette I from hNET, whereas chimera B corresponds to the reverse construct.2 The appearance or the intensity of a Cl -independent component of transport was linked to the presence of the COOH terminal part of hNET in both monoclonal and polyclonal Ltk cells (Cl- substituted by isethionate and NO3-,respectively), and in transiently transfected COS-7 cells.3 Cassette I was also involved in the Cl- -dependence because the transport activity of polyclonal Ltk cells expressing A was partly Cl--independent and because Ltk(-) cells expressing transporters containing cassette I of hDAT displayed higher K-mCl- values than cells expressing the reverse constructs.4 In monoclonal Ltk(-) cell lines, K-mNa+ values and biphasic vs monophasic dependence upon Na+ concentrations differentiate transporters containing cassette I of hNET from those containing cassette I of hDAT. Tn COS-7 cells, the exchange of cassette I produced a significant change in Hill number values.5 In Na+-dependence studies, exchange of the COOH terminal part significantly modified Hill number values in both Ltk and COS-7 cells.6 Hill number values close to two were found for hNET and hDAT when sucrose was used as substitute for NaCl.7 The NH2 terminal part of the transporters bears some of the differences in the Na+ and Cl--dependence of the uptake that are observed between hDAT and hNET. Present results also support a role of the: COOH terminal part inthe ionic dependence. British Journal of Pharmacology (2001).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:57:07