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Titolo:
Cloning of a lymphatic peptide/histidine transporter
Autore:
Sakata, K; Yamashita, T; Maeda, M; Moriyama, Y; Shimada, S; Tohyama, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Anat & Neurosci, Osaka 5650871, Japan OsakaUniv Osaka Japan 5650871 ept Anat & Neurosci, Osaka 5650871, Japan Core Res Evolut Sci & Technol, Kawagutchi 3320012, Japan Core Res Evolut Sci & Technol Kawagutchi Japan 3320012 hi 3320012, Japan Max Planck Inst Neurobiol, Dept Neurobiochem, D-82152 Martinsried, GermanyMax Planck Inst Neurobiol Martinsried Germany D-82152 rtinsried, Germany Osaka City Univ, Sch Med, Dept Anat 1, Abeno Ku, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 at 1, Abeno Ku, Osaka 5458585, Japan Okayama Univ, Fac Pharmaceut Sci, Dept Biochem, Okayama 7008530, Japan Okayama Univ Okayama Japan 7008530 Dept Biochem, Okayama 7008530, Japan Nagoya City Univ, Sch Med, Dept Anat 2, Mizuho Ku, Nagoya, Aichi 4670001, Japan Nagoya City Univ Nagoya Aichi Japan 4670001 Nagoya, Aichi 4670001, Japan
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 356, anno: 2001,
parte:, 1
pagine: 53 - 60
SICI:
0264-6021(20010515)356:<53:COALPT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBOXYL CYTOPLASMIC TAIL; PROTEIN LIMP-II; OLIGOPEPTIDE TRANSPORTER; H+/PEPTIDE COTRANSPORTER; CHROMOSOME 11Q12-Q13.1; PEPTIDE TRANSPORTER; EXPRESSION; LYSOSOMES; ARABIDOPSIS; REGION;
Keywords:
cellular localization; lysosome; macrophage; molecular cloning; subcellular localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Sakata, K NICHD, Unit Synapse Dev & Plast, LCSN, Bldg 49,Rm 6A67,49 Convent Dr,MSC 4480, Bethesda, MD 20892 USA NICHD Bldg 49,Rm 6A67,49 Convent Dr,MSC 4480 Bethesda MD USA 20892
Citazione:
K. Sakata et al., "Cloning of a lymphatic peptide/histidine transporter", BIOCHEM J, 356, 2001, pp. 53-60

Abstract

Although peptide transport across the plasma membrane has been characterized well in the kidney and the intestine, the functional relevance of this transport in other organs has not been addressed. Here we report the cloningof a cDNA for a novel peptide/histidine transporter found in the rat (rPHT2), whose mRNA is expressed mainly in the lymphatic system. rPHT2 encodes aprotein of 582 amino acids and showed 49% identity with the brain PHT (PHT1) [Yamashita, Shimada, Guo, Sate, Kohmura, Hayakawa, Takagi and Tohyama (1997) J. Biol, Chem. 272, 10205-10211]. rPHT2 mRNA was abundant in lung, spleen and thymus, and detected faintly in brain, liver, adrenal gland and heart by Northern-blot analysis and reverse transcriptase PCR (RT-PCR). Intense signals for the gene were found in immunocytes using in situ hybridization. Ectopic expression of rPHT2 protein in HEK-293T cells and BHK cells was nor found on the cell surface, but was found on the lysosomal membrane using light- and electron-microscopic analysis, Recombinant rPHT2 protein reconstituted into liposomes showed proton-dependent transport activity with histidine and histidyl-leucine, These findings suggest that rPHT2 is involved in the protein catabolic pathway in the lymphatic system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:11:31