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Titolo:
AG-041R, a gastrin/CCK-B antagonist, stimulates chondrocyte proliferation and metabolism in vitro
Autore:
Ochi, M; Kawasaki, K; Kataoka, H; Uchio, Y; Nishi, H;
Indirizzi:
Shimane Med Univ, Dept Orthopaed, Izumo, Shimane 6938501, Japan Shimane Med Univ Izumo Shimane Japan 6938501 zumo, Shimane 6938501, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 5, volume: 283, anno: 2001,
pagine: 1118 - 1123
SICI:
0006-291X(20010525)283:5<1118:AAGASC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; CHONDROITIN SULFATE; ARTICULAR-CARTILAGE; IN-VIVO; MATRIX; RATS; GEL;
Keywords:
cholecyctokinin-B/gastrin receptor antagonist; chondrocyte; articular cartilage; proliferation; chondroitin sulfate; collagen gel culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ochi, M Shimane Med Univ, Dept Orthopaed, 89-1 Enya Cho, Izumo, Shimane 6938501, Japan Shimane Med Univ 89-1 Enya Cho Izumo Shimane Japan 6938501 , Japan
Citazione:
M. Ochi et al., "AG-041R, a gastrin/CCK-B antagonist, stimulates chondrocyte proliferation and metabolism in vitro", BIOC BIOP R, 283(5), 2001, pp. 1118-1123

Abstract

A newly synthesized compound, AG-041R, 3R-1-(2,2Diethoxyethyl)-3- ((4methylphenyl) amino-carbonylmethyl)-3-( (4methylphenyl)ureido-indoline-2-one), is a cholecyctokinin-B/gastrin receptor antagonist, but unexpectedly magnified cartilage formation in vivo, Indeed, AG-041R is a potentially effective reagent for the repair of articular cartilage defects. To clarify its effects on chondrocytes, we studied the proliferation, matrix formation, and gene expression of rabbit primary chondrocytes cultured in type I collagen gelcomposites with AG-041R, Both proliferation and glycosaminoglycan synthesis were stimulated with 1 muM AG-041R, but suppressed with 10 muM. The ratioof the amounts of two chondroitin sulfate isomers, chondroitin-6-sulfate to chondroitin-4-sulfate tan indicator of cartilage maturation), increased with 1 muM but decreased with 10 muM AG-041R, Gene expression analysis showed there was no change in the relative expression levels of chondrocyte markers, Type II collagen and Aggrecan, and osteoblast and adipocyte markers, Type I collagen and PPAR gamma, respectively. These findings suggest that adequate concentrations of AG-041R stimulate proliferation of chondrocytes inthe matrix, without changing their differentiated characteristics. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 07:02:56