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Titolo:
Possible role of salsolinol quinone methide in the decrease of RCSN-3 cellsurvival
Autore:
Martinez-Alvarado, P; Dagnino-Subiabre, A; Paris, I; Metodiewa, D; Welch, CJ; Olea-Azar, C; Caviedes, P; Caviedes, R; Segura-Aguilar, J;
Indirizzi:
Univ Chile, ICBM, Program Mol & Clin PHarmacol, Fac Med, Santiago 7, ChileUniv Chile Santiago Chile 7 & Clin PHarmacol, Fac Med, Santiago 7, Chile Univ Chile, Fac Chem & Pharmacol Sci, Dept Analyt Chem, Santiago 7, Chile Univ Chile Santiago Chile 7 col Sci, Dept Analyt Chem, Santiago 7, Chile Lodz Tech Univ, Inst Appl Radiat Chem, PL-93590 Lodz, Poland Lodz Tech Univ Lodz Poland PL-93590 l Radiat Chem, PL-93590 Lodz, Poland Uppsala Univ, Biomed Ctr, Div Organ Pharmaceut Chem, Uppsala, Sweden Uppsala Univ Uppsala Sweden Div Organ Pharmaceut Chem, Uppsala, Sweden Sansum Med Res Inst, Santa Barbara, CA USA Sansum Med Res Inst Santa Barbara CA USA Res Inst, Santa Barbara, CA USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 5, volume: 283, anno: 2001,
pagine: 1069 - 1076
SICI:
0006-291X(20010525)283:5<1069:PROSQM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE; RAT-LIVER; DOPAMINE; AMINOCHROME; PEROXIDASE; OXIDATION; CATALASE; BRAIN; CDNA; 1(R),2(N)-DIMETHYL-6,7-DIHYDROXY-1,2,3,4-TETRAHYDROISOQUINOLINE;
Keywords:
salsolinol; quinone methide; dopamine; DAT; DT-diaphorase; lactoperoxidase; dicoumarol; catalase; glutathione peroxidase; substantia nigra;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Segura-Aguilar, J Univ Chile, ICBM, Program Mol & Clin PHarmacol, Fac Med,Independencia 1027,Casilla 70000, Santiago 7, Chile Univ Chile Independencia 1027,Casilla 70000 Santiago Chile 7
Citazione:
P. Martinez-Alvarado et al., "Possible role of salsolinol quinone methide in the decrease of RCSN-3 cellsurvival", BIOC BIOP R, 283(5), 2001, pp. 1069-1076

Abstract

The endogenous dopamine-derived neurotoxin salsolinol was found to decrease survival in the dopaminergic neuronal cell line RCSN-3, derived from adult rat substantia nigra in a concentration-dependent manner (208 muM salsolinol induced a 50% survival decrease). Incubation of RCSN-3 cells with 100 muM dicoumarol and salsolinol significantly decreased cell survival by 2.5-fold (P < 0.001), contrasting with a negligible effect on RCHT cells, which exhibited nearly a 5-fold lower nomifensine-insensitive dopamine uptake. The levels of catalase and glutathione peroxidase mRNA were decreased when RCSN-3 cells were treated with 100 <mu>M salsolinol alone or in the presence of 100 muM dicoumarol, In vitro oxidation of salsolinol to o-quinone catalyzed by lactoperoxidase gave the quinone methide and 1,2-dihydro-1-methyl-6,7-isoquinoline diol as final products of salsolinol oxidation as determinedby NMR analysis. Evidence of the formation of salsolinol o-semiquinone radical has been provided by ESR studies during one-electron oxidation of salsolinol catalyzed by lactoperoxidase. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:44:02