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Titolo:
Changes in vimentin in human macrophages during apoptosis induced by oxidised low density lipoprotein
Autore:
Muller, K; Dulku, S; Hardwick, SJ; Skepper, JN; Mitchinson, MJ;
Indirizzi:
Univ Cambridge, Dept Pathol, Div Cellular Mol Pathol, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP hol, Cambridge CB2 1QP, England Univ Cambridge, Dept Anat, Multiimaging Ctr, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP Ctr, Cambridge CB2 1QP, England
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 1, volume: 156, anno: 2001,
pagine: 133 - 144
SICI:
0021-9150(200105)156:1<133:CIVIHM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCYTE-MACROPHAGES; SMOOTH-MUSCLE CELLS; INTERMEDIATE FILAMENTS; ADIPOSE CONVERSION; CALPAIN ACTIVATION; OXIDIZED LDL; LIPID CORE; CHOLESTEROL; ATHEROSCLEROSIS; DEATH;
Keywords:
atherosclerosis; apoptosis; macrophages (human); vimentin; oxidised low density lipoprotein; caspases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, K Univ Cambridge, Dept Pathol, Div Cellular Mol Pathol, Tennis Court Rd, Cambridge CB2 1QP, England Univ Cambridge Tennis Court Rd CambridgeEngland CB2 1QP ngland
Citazione:
K. Muller et al., "Changes in vimentin in human macrophages during apoptosis induced by oxidised low density lipoprotein", ATHEROSCLER, 156(1), 2001, pp. 133-144

Abstract

Macrophage apoptosis contributes to the development of human atherosclerotic lesions. Oxidised LDL may be involved in macrophage death in vivo. We examined morphological and biochemical changes to the vimentin filament network during apoptosis of human macrophages. Only oxidised LDL. but not nativeor acetylated LDL, induced apoptosis, wherein vimentin was cleaved into fragments of 48-50, 46, 29 and 26 kDa. The use of caspase inhibitors suggested that caspase-6 mediates the formation of the 26 and 46 kDa fragments of vimentin. We were unable to demonstrate any significant involvement of caspase-3 in vimentin cleavage. However, caspase-3 was clearly activated during apoptosis whilst caspase-6 expression in macrophages was minimal. Vimentin filament breakdown occurred early during apoptosis and vimentin immunoreactivity was present in apoptotic bodies. However, the application of caspase inhibitors had no effect on the morphology of the vimentin network in apoptotic cells. suggesting that filament breakdown is not mediated by caspase proteolysis. Similar changes in vimentin were also seen in gliotoxin-inducedapoptosis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:41:05