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Titolo:
Defibrinogenating effect of batroxobin (Defibrase (R)) in rats and inhibition of migration of human vascular smooth muscle cells by the plasma of batroxobin-treated rats in vitro
Autore:
Wang, DS; Hanamoto, M; Fang, F; Ohba, M; Ishii, M; Kimura, F; Higaki, E; Senga, H;
Indirizzi:
Tobishi Pharmaceut Co Ltd, Ome Res Labs, Ome, Tokyo 1980025, Japan TobishiPharmaceut Co Ltd Ome Tokyo Japan 1980025 e, Tokyo 1980025, Japan
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 1, volume: 156, anno: 2001,
pagine: 73 - 80
SICI:
0021-9150(200105)156:1<73:DEOB((>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEGRADATION PRODUCTS; FIBRINOGEN; GROWTH; ATHEROSCLEROSIS;
Keywords:
batroxobin (Defibrase (R)); fibrinogen; ELISA; rat plasma; rat serum; human vascular smooth muscle cell; cell migration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, DS Tobishi Pharmaceut Co Ltd, Ome Res Labs, 7-1-1 Chome, Ome, Tokyo 1980025, Japan Tobishi Pharmaceut Co Ltd 7-1-1 Chome Ome Tokyo Japan 1980025 pan
Citazione:
D.S. Wang et al., "Defibrinogenating effect of batroxobin (Defibrase (R)) in rats and inhibition of migration of human vascular smooth muscle cells by the plasma of batroxobin-treated rats in vitro", ATHEROSCLER, 156(1), 2001, pp. 73-80

Abstract

The defibrinogenating effect of batroxobin (Defibrase(R)) in male Wistar rats and the inhibitory effects of the plasma of batroxobin-treated rats on the migration of human vascular smooth muscle cells (SMCs) were investigated in vitro. At 1 h after a single intravenous injection of 3.0, 10.0 or 30.0 BU/kg batroxobin (ten rats in each group), the fibrinogen levels in the plasma of the rats decreased to 88.3, 66.2 and 16.5%. respectively. of that in the plasma of control saline-treated rats (261.0 +/- 26.7 mg/dl). When the plasma from the batroxobin-treated rats was added to Dulbecco's modifiedEagle's medium at a concentration of 0.2% for a vascular SMC migration assay and incubated in a modified Boyden's chamber system at 37 degreesC for 24 h, significant inhibitory effects on vascular SMC migration were observedin the 10.0 (P < 0.05) and 30.0 BU/kg (P < 0.01) batroxobin-treated rats. The plasma of batroxobin-treated rats as well as standard rat fibrinogen induced vascular SMC migration in a fibrinogen content-dependent manner except the plasma of the 30.0 BU/kg batroxobin-treated rats. Moreover, the rat serum (0.1 similar to 5.0%) did not show any activity on vascular SMC migration in the present experimental system. These results indicate that the plasma fibrinogen significantly influences vascular SMC migration, and that the inhibitory effect of the plasma of batroxobin-treated rats on vascular SMC migration is related to the defibrinogenating action of batroxobin in vivo. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 06:59:50