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Titolo:
Lepidopteran peritrophic membranes and effects of dietary wheat germ agglutinin on their formation and structure
Autore:
Hopkins, TL; Harper, MS;
Indirizzi:
Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 Entomol, Manhattan, KS 66506 USA
Titolo Testata:
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
fascicolo: 2, volume: 47, anno: 2001,
pagine: 100 - 109
SICI:
0739-4462(200106)47:2<100:LPMAEO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASEOLUS-VULGARIS; OSTRINIA-NUBILALIS; PLANT-LECTINS; MECHANISM; LARVAE; SECRETION;
Keywords:
peritrophic membrane; chitin; midgut; microscopy; wheat germ agglutinin; lectins; European corn borer; Ostrinia nubilalis; tobacco hornworm; Manduca sexta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Hopkins, TL Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 hattan, KS 66506 USA
Citazione:
T.L. Hopkins e M.S. Harper, "Lepidopteran peritrophic membranes and effects of dietary wheat germ agglutinin on their formation and structure", ARCH INS B, 47(2), 2001, pp. 100-109

Abstract

Peritrophic membrane (PM) structure and the effects of dietary wheat germ agglutinin (WGA) on PM formation were studied in larvae of the European corn borer (ECB), Ostrinia nubilalis, and the tobacco hornworm (THW), Manduca sexta. Growth of ECB was strongly inhibited by low amounts of WGA in the diet (0.05%), whereas THW was not affected by amounts of up to 2%. In ECB larvae, chitin microfibrils were secreted to form an orthogonal network withinthe apical region of the anterior midgut microvilli. The network then moved to the tips of the microvilli where proteinacious matrix was added prior to delamination of a single PM into the lumen to enclose the food bolus. Multiple PMs rapidly appeared as the food moved posteriorly and some of thesebecame greatly thickened in the middle and posterior regions of the midgut. WGA in the diet caused hypersecretion of unorganized PM in the anterior midgut lumen, disintegration of microvilli, and cessation of feeding. It wasalso shown to bind to both the chitinous network and to several PM proteins, perhaps causing voids in the PM and sparse matrix material. This allowedthe passage of food particles through a defective PM into the ectoperitrophic space and penetration into the microvillar brush border. Stimulation ofPM secretion and cessation of feeding may have been ah response to damage to the brush border. Unlike ECE, the chitinous network of THW is a randomlyorganized felt-like structure embedded in a proteinaceous matrix. This PM is secreted as a thin multilayered structure in the anterior region of the midgut but multiple and thickened PMs occur in the middle and posterior lumens of the midgut. THW tolerated high amounts of WGA in its diet with no disruption of PM formation or inhibition of growth. WGA did accumulate as large masses embedded in the PM, but caused no voids that would allow the penetration of food particles and subsequent damage to the brush border. Therefore, differences in PM formation and structure between ECB and THW appearedto affect how WGA interacts with chitinous and proteinaceous components ofthe PM and subsequent effects on larval feeding and growth. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:20:08