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Titolo:
GM-CSF downmodulates c-kit, Fc epsilon RI alpha and GM-CSF receptor expression as well as histamine and tryptase levels in cultured human mast cells
Autore:
Welker, P; Grabbe, J; Zuberbier, T; Grutzkau, A; Henz, BM;
Indirizzi:
Humboldt Univ, Charite, Dept Dermatol & Allergy, D-13344 Berlin, Germany Humboldt Univ Berlin Germany D-13344 & Allergy, D-13344 Berlin, Germany Med Univ Lubeck, Dept Dermatol, D-23538 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23538 Dermatol, D-23538 Lubeck, Germany
Titolo Testata:
ARCHIVES OF DERMATOLOGICAL RESEARCH
fascicolo: 5, volume: 293, anno: 2001,
pagine: 249 - 258
SICI:
0340-3696(200105)293:5<249:GDCFER>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; IMMUNOSTIMULATORY DENDRITIC CELLS; CORD BLOOD-CELLS; INDEPENDENT ACTIVATION; MONOCLONAL-ANTIBODIES; RECOMBINANT HUMAN; PROGENITOR CELLS; LINE HMC-1; TNF-ALPHA; LIGAND;
Keywords:
GM-CSF; mast cells; c-kit; Fc epsilon RI; TrkA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Welker, P Humboldt Univ, Charite, Dept Dermatol & Allergy, Augustenburger Pl 1, D-13344 Berlin, Germany Humboldt Univ Augustenburger Pl 1 Berlin Germany D-13344 ermany
Citazione:
P. Welker et al., "GM-CSF downmodulates c-kit, Fc epsilon RI alpha and GM-CSF receptor expression as well as histamine and tryptase levels in cultured human mast cells", ARCH DERM R, 293(5), 2001, pp. 249-258

Abstract

GM-CSF is known primarily as a hematopoietic growth factor, but it has also been shown to inhibit mast cell differentiation in vitro. In order elucidate the mechanisms involved, we investigated the effects of GM-CSF in vitroon the differentiation of human leukemic mast cells (HMC-1 cells) and normal cord blood-derived mast cells (CBMC) under the influence of SCF, NGF, and fibroblast supernatant (FS). Under all culture conditions, GM-CSF induceda dose-and time-dependent reduction in intracellular histamine levels, tryptase activity, and numbers of cells immunoreactive for c-Kit and Fc epsilon RI alpha, This effect leveled off between 10-100 ng/ml and after 4 days of culture. There was an associated decrease in mRNA expression for c-kit, Fc epsilon RI alpha and tryptase, In contrast, no significant changes in theexpression of the NGF receptor TrkA were noted under the same conditions. The GMCSF receptor was found in HMC-1 cells and CBMC at both the mRNA and protein levels, but its expression decreased during culture with FS, and even more markedly during culture with GM-CSF, GM-CSF thus selectively inhibits in vitro induction and/or upregulation of all major mast cell characteristics in HMC-1 cells and CBMC irrespective of the growth factors present, and a concomitant downregulation of GM-CSF receptors can counteract these effects. GM-CSF may therefore function as a regulatory factor in mast cell growth and differentiation under normal and pathological conditions.

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Documento generato il 09/04/20 alle ore 20:00:59