Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of the butylated hydroxyanisole and butylated hydroxytoluene on the DNA adduct formation and arylamines N-acetyltransferase activity in humancolon tumor cells
Autore:
Chang, SH; Chen, GW; Yeh, CC; Hung, CF; Lin, SS; Chung, JG;
Indirizzi:
China Med Coll, Dept Microbiol, Taichung 400, Taiwan China Med Coll Taichung Taiwan 400 Dept Microbiol, Taichung 400, Taiwan China Med Coll, Dept Surg, Taichung 400, Taiwan China Med Coll Taichung Taiwan 400 Coll, Dept Surg, Taichung 400, Taiwan China Med Coll, Dept Urol, Taichung 400, Taiwan China Med Coll Taichung Taiwan 400 Coll, Dept Urol, Taichung 400, Taiwan Jen Ai Hosp, Dept Surg, Taichung, Taiwan Jen Ai Hosp Taichung TaiwanJen Ai Hosp, Dept Surg, Taichung, Taiwan Chung Tai Inst Hlth Sci & Technol, Dept Radiol Technol, Taichung, Taiwan Chung Tai Inst Hlth Sci & Technol Taichung Taiwan nol, Taichung, Taiwan
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 2A, volume: 21, anno: 2001,
pagine: 1087 - 1093
SICI:
0250-7005(200103/04)21:2A<1087:EOTBHA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLADDER-CANCER; HUMAN LIVER; CHEMICAL CARCINOGENS; COLORECTAL-CANCER; ACETYLATION; 2-AMINOFLUORENE; ACTIVATION; ANTIOXIDANTS; EXPRESSION; ENHANCEMENT;
Keywords:
butylated hydroxyanisole; butylated hydroxy-toluene; DN A adduct formation; N- acetyltransferase; 2-aminofluorene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, JG China Med Coll, Dept Med, 91 Hsueh Shih Rd,, Taichung 400, Taiwan China Med Coll 91 Hsueh Shih Rd, Taichung Taiwan 400 00, Taiwan
Citazione:
S.H. Chang et al., "Effects of the butylated hydroxyanisole and butylated hydroxytoluene on the DNA adduct formation and arylamines N-acetyltransferase activity in humancolon tumor cells", ANTICANC R, 21(2A), 2001, pp. 1087-1093

Abstract

The effects of butylated hydroxyanisole (BHA) and butylated hydroxytoluene(BHT) on N-acetyltransferase (NAT) activity were examined using a human colon tumor cell line (colo 205). BHA or BHT were added to the cytosols or tothe medium of human colon rumor cells: The NAT activity was measured by high performance liquid chromatography, assaying the amounts of acetylated 2-aminofluorene (AAF), p aminobenzoic acid (N-Ac-PABA), nonacetylated 2 amino-fluorene (AF) and p-aminobenzoic acid (PABA). The NAT activity in the human colon tumor cells and cytosols was suppressed by BHA or BHT in a close-dependent manner. The apparent values of Km and Vmax of NAT of human colon tumor cells were also decreased by BHA or BHT in cytosols and in intact cells. BHA ol BHT may act as a noncompetitive mhibitor. After the incubation of human colon tumor cells with AF in the presence of BHA or BHT, the cells were recovered and DNA was prepared and hydrolysed to nucleotides. Adducted nucleotides were extracted into butanol and AF-DNA adducts were analysed by HPLC. The results also demonstrated that when BHA or BHT was added to the media, a decrease in AF-DNA adduct formation was seen in the human colon tumor cells. The finding of AF-DNA adduct formation in cultured human colon tumor cells suggest the possibility of using cultured cells for assessing arylamine-induced DNA damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:26:40