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Titolo:
Modulation of multidrug resistance in a cancer cell line by anti-multidrugresistance-associated protein (MRP) ribozyme
Autore:
Hatanaka, H; Abe, Y; Naruke, M; Asai, S; Miyachi, H; Kawakami, T; Nagata, J; Kamochi, JI; Kijima, H; Yamazaki, H; Scanlon, KJ; Ueyama, Y; Nakamura, M;
Indirizzi:
Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Clin Pathol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Natl Sanatorium Kanagawa Hosp, Dept Resp Dis, Kanagawa 2578585, Japan NatlSanatorium Kanagawa Hosp Kanagawa Japan 2578585 gawa 2578585, Japan Keck Grad Inst, Claremont, CA 91711 USA Keck Grad Inst Claremont CA USA 91711 Grad Inst, Claremont, CA 91711 USA
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 2A, volume: 21, anno: 2001,
pagine: 879 - 885
SICI:
0250-7005(200103/04)21:2A<879:MOMRIA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PANCREATIC-CANCER; LUNG-CANCER; GENE-EXPRESSION; MESSENGER-RNA; HAMMERHEAD RIBOZYMES; DRUG-RESISTANCE; P53 PROTEIN; CARCINOMA; GROWTH; DNA;
Keywords:
MRP; ribozyme; multidrug resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamura, M Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 gawa 2591193, Japan
Citazione:
H. Hatanaka et al., "Modulation of multidrug resistance in a cancer cell line by anti-multidrugresistance-associated protein (MRP) ribozyme", ANTICANC R, 21(2A), 2001, pp. 879-885

Abstract

Multidrug resistance-associated protein (MRP) is the major candidate molecule responsible for non-P-glycoprotein (P-Gp)-mediated multidrug resistance. We used a hammerhead anti-MRP ribozyme (alpha MRP-Rz) to inactivate MRP function in a multidrug resistant cancer cell line, KB8-5. The B-actin promoter-driven aMRP-Rz sequence (pH beta/alpha MRP-Rz) was introduced into KB8-5 cells (KB8-5/alpha MRP-Rz) and we evaluated growth of the cell line. The gene expression of multidrug resistance-related molecules was estimated. Drug sensitivity was estimated by MTT assay in vitro. MRP mRNA expression wasdecreased in KB8-5/alpha MRP-Rz cells. The MTT assay showed increased IC50values or resistance to doxorubicin (DOX), etoposide (VP-16) and cisplatin(CDDP) in KB8-5/alpha MRP-Rz cells. No significant differences were observed in expression of multidrug resistance gene (MDR1), thymidylate synthase,glutathione S-transferase pi or topoisomerase IIa. The hammerhead ribozyme-mediated simple suppression of MRP mRNA expression was not sufficient to reverse multidrug resistance in the cancer cell line KB8-5.

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Documento generato il 09/04/20 alle ore 10:47:08