Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Enhanced maturation of porcine neonatal pancreatic cell clusters with growth factors fails to improve transplantation outcome
Autore:
Lopez-Avalos, MD; Tatarkiewicz, K; Sharma, A; Bonner-Weir, S; Weir, GC;
Indirizzi:
Joslin Diabet Ctr, Div Res, Boston, MA 02215 USA Joslin Diabet Ctr BostonMA USA 02215 Ctr, Div Res, Boston, MA 02215 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA Harvard Univ BostonMA USA 02215 Sch Med, Dept Med, Boston, MA 02215 USA
Titolo Testata:
TRANSPLANTATION
fascicolo: 8, volume: 71, anno: 2001,
pagine: 1154 - 1162
SICI:
0041-1337(20010427)71:8<1154:EMOPNP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FETAL-RAT ISLETS; BETA-CELLS; NUDE-MICE; SODIUM-BUTYRATE; FACTOR-I; PLACENTAL-LACTOGEN; INSULIN-SECRETION; DIABETES-MELLITUS; FACTOR RECEPTORS; SCATTER FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Weir, GC Joslin Diabet Ctr, Div Res, Boston, MA 02215 USA Joslin Diabet Ctr Boston MA USA 02215 Res, Boston, MA 02215 USA
Citazione:
M.D. Lopez-Avalos et al., "Enhanced maturation of porcine neonatal pancreatic cell clusters with growth factors fails to improve transplantation outcome", TRANSPLANT, 71(8), 2001, pp. 1154-1162

Abstract

Background. Porcine neonatal pancreatic cell clusters (NPCC) are a potential source of islet tissue for clinical transplantation. They can normalize glycemia after transplantation, although after a relatively long (several weeks) period of time, possibly due to the immaturity of the tissue. Methods. One week after isolation NPCCs were immobilized in alginate hydrogel to be cultured for 2 more weeks in the presence of different growth factors, which were applied individually or in various combinations. Their effect was assessed by measuring DNA and insulin content, and expression of islet genes by reverse transcriptase-polymerase chain reaction. Enhanced maturation of NPCCs was also evaluated after transplantation in streptozotocin-diabetic mice. Results, A combination of fetal calf serum, insulin-like growth factor-I, nicotinamide and sodium butyrate in NPCCs media from day 7 to day 21 resulted in increased insulin/DNA content and higher expression of insulin, somatostatin, GLUT2 and Nkx6.1 genes. NPCCs cultured under the same conditions from day 3 to day 12 were transplanted into diabetic mice. Control mice weretransplanted with NPCCs cultured in parallel in the presence of nicotinamide, but with no serum, insulin-like growth factor-I or butyrate. Normoglycemia was achieved at the same rate in both groups. Plasma porcine C-peptide (week 6) and graft insulin content (week 20) were also similar in both groups. Conclusions. Increased insulin content of NPCCs was achieved in vitro by addition of fetal calf serum, insulin-like growth factor-I, nicotinamide, and sodium butyrate, but this increase did not translate into a faster achievement of normoglycemia after transplantation, which suggests that there is a time frame required for complete maturation that is difficult to alter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:19:21