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Titolo:
Isolation and sequence determination of peptides in the venom of the spider wasp (Cyphononyx dorsalis) guided by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry
Autore:
Konno, K; Hisada, M; Naoki, H; Itagaki, Y; Yasuhara, T; Juliano, MA; Juliano, L; Palma, MS; Yamane, T; Nakajima, T;
Indirizzi:
Sao Paulo State Univ, Inst Biosci Rio Claro, Ctr Study Social Insects, BR-13506900 Rio Claro, Brazil Sao Paulo State Univ Rio Claro Brazil BR-13506900 BC00 Rio Claro, Brazil Suntory Inst Bioorgan Res, Shimamoto, Osaka 6188503, Japan Suntory Inst Bioorgan Res Shimamoto Osaka Japan 6188503 ka 6188503, Japan Columbia Univ, Dept Chem, New York, NY 10027 USA Columbia Univ New York NY USA 10027 iv, Dept Chem, New York, NY 10027 USA Tokyo Univ Agr, Jr Coll Agr, Dept Nutr, Setagaya Ku, Tokyo 1568502, Japan Tokyo Univ Agr Tokyo Japan 1568502 tr, Setagaya Ku, Tokyo 1568502, Japan Inst Butantan, Mol Toxicol Lab, BR-05503900 Sao Paulo, Brazil Inst Butantan Sao Paulo Brazil BR-05503900 BC-05503900 Sao Paulo, Brazil
Titolo Testata:
TOXICON
fascicolo: 8, volume: 39, anno: 2001,
pagine: 1257 - 1260
SICI:
0041-0101(200108)39:8<1257:IASDOP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTOXINS; KININS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Konno, K Sao Paulo State Univ, Inst Biosci Rio Claro, Ctr Study Social Insects, BR-13506900 Rio Claro, Brazil Sao Paulo State Univ Rio Claro BrazilBR-13506900 BCaro, Brazil
Citazione:
K. Konno et al., "Isolation and sequence determination of peptides in the venom of the spider wasp (Cyphononyx dorsalis) guided by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry", TOXICON, 39(8), 2001, pp. 1257-1260

Abstract

Micro-scale (sub-pmol) isolation and sequence determination of three peptides from the venom of the solitary spider wasp Cyphononyx dorsalis is described. We isolated two novel peptides Cd-125 and Cd-146 and a known peptide Thr(6)-bradykinin from only two venom sacs of solitary spider wasp Cyphononyx dorsalis without bioassay-guided fractionation. but instead guided by MALDI-TOF MS. The MALDI-TOF MS analysis of each fraction showed the purity and molecular weight of the components, which led to the isolation of the peptides virtually without loss of sample amount. The sequences of the novel peptides Cd-125 (Asp-Thr-Ala-Arg-Leu-Lys-Trp-His) and Cd-146 (Ser-Glu-Thr-Gly-Asn-Thr-Val-Thr-Val-Lys-Gly-Phe-Ser-Pro-Leu-Arg) were determined by Edmandegradation together with mass spectrometry. and finally corroborated by solid-phase synthesis. The known peptide Thr(6)-bradykinin (Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg) was identified by comparison with the synthetic authentic specimen. This is the first example for any kinins to be found in Pompilidae wasp venoms. The procedure reported here can be applicable to studieson many other components of solitary wasp venoms with limited sample availability. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/18 alle ore 08:39:04