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Titolo:
Photosensitive chemical and laser light treatments decrease epizootic hemorrhagic disease virus associated with in vitro produced bovine embryos
Autore:
Dinkins, MB; Stallknecht, DE; Howerth, EW; Brackett, BG;
Indirizzi:
Univ Georgia, Coll Vet Med, Dept Physiol & Pharmacol, Athens, GA 30602 USAUniv Georgia Athens GA USA 30602 hysiol & Pharmacol, Athens, GA 30602 USA Univ Georgia, Coll Vet Med, SE Cooperat Wildlife Dis Study, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 Wildlife Dis Study, Athens, GA 30602 USA Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 t Med, Dept Pathol, Athens, GA 30602 USA
Titolo Testata:
THERIOGENOLOGY
fascicolo: 8, volume: 55, anno: 2001,
pagine: 1639 - 1655
SICI:
0093-691X(20010501)55:8<1639:PCALLT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRAL DIARRHEA VIRUS; PREIMPLANTATION MOUSE EMBRYO; INNER CELL MASS; IN-VITRO; TRYPSIN TREATMENT; IVF EMBRYOS; ZONA-INTACT; OOCYTES; HERPESVIRUS-1; GLUTATHIONE;
Keywords:
bovine; embryo; IVF; virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Brackett, BG Univ Georgia, Coll Vet Med, Dept Physiol & Pharmacol, Athens,GA 30602 USA Univ Georgia Athens GA USA 30602 macol, Athens, GA 30602 USA
Citazione:
M.B. Dinkins et al., "Photosensitive chemical and laser light treatments decrease epizootic hemorrhagic disease virus associated with in vitro produced bovine embryos", THERIOGENOL, 55(8), 2001, pp. 1639-1655

Abstract

Photoinactivation was employed to eliminate EHDV-2 from in vitro produced bovine embryos experimentally exposed to this virus. Immature oocytes were matured, fertilized, and cultured in chemically defined conditions. All treatments were performed on zygotes. Developmental potential of zygotes and cell numbers of resulting hatched blastocysts were assessed after exposure to a 1 mW helium neon laser (633 nm, red) for 1, 5, 10, and 15 min; the photosensitive chemicals hematoporphyrin (15 muM) and hypericin (1 and 10 muM) for 15 min; a combination of 10 muM hypericin and laser light for 1, 3, or 5 min; and a combination of 15 muM hematoporphyrin and laser light for 1, 2, or 3 min. There were no significant differences among proportions of embryos developing or cell numbers after treatment with or without exposure to laser light alone for up to 10 min. No differences were observed after exposure of zygotes to photosensitive chemicals alone. Exposure to 10 muM hypericin and 5 min of laser light or 15 muM hematoporphyrin and 2 min of laser light compromised zygote developmental potential. After exposure to 10(6) TCID50/mL EHDV-2 for 90 min groups of 10 zygotes were exposed to 10 muM hypericin or 15 muM hematoporphyrin and laser light to inactivate the virus. Hematoporphyrin was effective with 3 min light exposure at reducing the percentage of EHDV-2 contaminated zygote pools (16.7%) as compared to EKDV-2 exposed pools without treatment (88.9%) but hematoporphyrin + 1 min light was ineffective. intermediate effect (55.6%). (C) 2001 by Elsevier Science Inc.

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Documento generato il 10/07/20 alle ore 08:51:50