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Titolo:
Crucial role of donor-derived stromal cells in successful treatment for intractable autoimmune diseases in MRL/lpr mice by BMT via portal vein
Autore:
Kushida, T; Inaba, M; Hisha, H; Ichioka, N; Esumi, T; Ogawa, R; Iida, H; Ikehara, S;
Indirizzi:
Kansai Med Univ, Transplantat Ctr, Dept Pathol 1, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan Kansai Med Univ, Dept Orthoped Surg, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan
Titolo Testata:
STEM CELLS
fascicolo: 3, volume: 19, anno: 2001,
pagine: 226 - 235
SICI:
1066-5099(2001)19:3<226:CRODSC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM-CELLS; RHEUMATOID-ARTHRITIS; VENOUS INJECTION; FELTYS-SYNDROME; LPR/LPR MICE; FLARE-UP; PREVENTION; INDUCTION;
Keywords:
stromal cells; MRL/lpr mouse; bone marrow transplantation; portal vein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Ikehara, S Kansai Med Univ, Transplantat Ctr, Dept Pathol 1, 10-15 Fumizono Cho, Moriguchi, Osaka 5708506, Japan Kansai Med Univ 10-15 Fumizono Cho Moriguchi Osaka Japan 5708506
Citazione:
T. Kushida et al., "Crucial role of donor-derived stromal cells in successful treatment for intractable autoimmune diseases in MRL/lpr mice by BMT via portal vein", STEM CELLS, 19(3), 2001, pp. 226-235

Abstract

We have recently established a new bone marrow transplantation (BMT) method for the treatment of intractable autoimmune diseases in MRL/lpr mice; themethod consists of fractionated irradiation (5.5 Gy x 2), followed by BMT of whole bone marrow cells (BMCs) from allogeneic C57BL/6 mice via the portal vein (abbreviated as 5.5 Gy x 2 + PV), In the present study, we investigate the mechanisms underlying the early engraftment of donor-derived cells in MRL/lpr mice by this method. In the mice treated with this method, the number of donor-derived cells possessing the mature lineage (Lin) markers rapidly increased in the BM, spleen, and Liver; almost 100% were donor-derived cells by 14 days after the treatment. The number of donor-derived hemopoietic progenitor cells (defined as c-kit(+)/Lin(-) cells) increased in the BMCs, hepatic mononuclear cells, and especially spleen cells by 14 days after the treatment. Simultaneously, hemopoietic foci adjoining donor-derived stromal cells were observed in the liver when injected via the PV, but not via the peripheral vein (i.v.), When adherent cell-depleted BMCs were injected via the PV, recipients showed a marked reduction in the survival rate. However, when mire were transplanted with adherent cell-depleted BMCs with cultured stromal cells, all the recipients survived. These findings suggest that not only donor hematopoietic stem cells (HSCs)but also donor stromal cells administered via the PV were trapped in the liver, resulting in the early engraftment of donor HSCs in cooperation with donor-derived stromal cells. This new strategy to facilitate the early recovery of hemopoiesis would therefore be of great advantage in human application.

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Documento generato il 04/04/20 alle ore 14:01:42