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Titolo:
Determination of 8-oxoguanine in individual cell nucleus of gamma-irradiated mammalian cells
Autore:
Chen, SK; Tsai, MH; Lin, CH; Hwang, JJ; Chan, WP;
Indirizzi:
Natl Yang Ming Univ, Sch Med, Inst Environm Hlth Sci, Taipei 11200, TaiwanNatl Yang Ming Univ Taipei Taiwan 11200 m Hlth Sci, Taipei 11200, Taiwan Natl Yang Ming Univ, Inst Publ Hlth, Taipei 11200, Taiwan Natl Yang Ming Univ Taipei Taiwan 11200 Publ Hlth, Taipei 11200, Taiwan Natl Yang Ming Univ, Inst Immunol & Microbiol, Taipei 11200, Taiwan Natl Yang Ming Univ Taipei Taiwan 11200 Microbiol, Taipei 11200, Taiwan Natl Yang Ming Univ, Inst Radiol Sci, Taipei 11200, Taiwan Natl Yang Ming Univ Taipei Taiwan 11200 Radiol Sci, Taipei 11200, Taiwan Taipei Municipal Jen Ai Hosp, Dept Family Med, Taipei, Taiwan Taipei Municipal Jen Ai Hosp Taipei Taiwan t Family Med, Taipei, Taiwan
Titolo Testata:
RADIATION RESEARCH
fascicolo: 6, volume: 155, anno: 2001,
pagine: 832 - 836
SICI:
0033-7587(200106)155:6<832:DO8IIC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; OXIDATIVE DAMAGE; DNA-DAMAGE; REPAIR; 8-HYDROXYDEOXYGUANOSINE; DERIVATIVES; BASES; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Chan, WP Natl Yang Ming Univ, Sch Med, Inst Environm Hlth Sci, 155 Sect,2 Lih Non St, Taipei 11200, Taiwan Natl Yang Ming Univ 155 Sect,2 Lih Non St Taipei Taiwan 11200 an
Citazione:
S.K. Chen et al., "Determination of 8-oxoguanine in individual cell nucleus of gamma-irradiated mammalian cells", RADIAT RES, 155(6), 2001, pp. 832-836

Abstract

8-Oxoguanine, through its ability to mispair bases other than cytosine, isassumed to be one of the most potent premutagenic lesions in nuclear DNA damaged by reactive oxygen radicals. In this study, we examine whether the presence of residual 8-oxoguanine can be detected in mammalian cells after exposure to ionizing radiation. MOLT-4 human leukemia cells and CHO-K1 Chinese hamster cells were acutely irradiated in vitro with 0, 0.2, 0.4, 0.6 and1.0 Gy gamma radiation at room temperature. The amounts of 8-oxoguanine and total DNA in the cell nucleus were detected by fluorescein-isothiocyanate(FITC)-labeled avidin, which binds specifically and directly to 8-oxoguanine, and propidium iodide, respectively. The intensity ratios between these two fluorescent dyes were then taken as indices to measure the content of 8-oxoguanine within individual cells. We found an apparent dose-dependent increase in the amount of 8-oxoguanine accumulated in cells of both lines. Moreover, the content of 8-oxoguanine decreased from 2 to 20 h after irradiation in CHO-K1 cells, which may reflect the time-dependent repair processes at the 8-oxoguanine lesions. This novel approach may provide a sensitive tool for in situ measurement of 8-oxoguanine in cells or even in the human body after exposure to ionizing radiation. (C) 2001 by Radiation Research Society.

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Documento generato il 02/10/20 alle ore 01:17:08