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Titolo:
The role of glutathione S-transferases in the detoxification of some organophosphorus insecticides in larvae and pupae of the yellow mealworm, Tenebrio molitor (Coleoptera : Tenebrionidae)
Autore:
Kostaropoulos, I; Papadopoulos, AI; Metaxakis, A; Boukouvala, E; Papadopoulou-Mourkidou, E;
Indirizzi:
Aristotelian Univ Salonika, Sch Biol, Dept Zool, Physiol Anim Lab, Thessaloniki 54006, Greece Aristotelian Univ Salonika Thessaloniki Greece 54006 oniki 54006, Greece Aristotelian Univ Salonika, Pesticide Sci Lab, Thessaloniki 54006, Greece Aristotelian Univ Salonika Thessaloniki Greece 54006 oniki 54006, Greece
Titolo Testata:
PEST MANAGEMENT SCIENCE
fascicolo: 6, volume: 57, anno: 2001,
pagine: 501 - 508
SICI:
1526-498X(200106)57:6<501:TROGSI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DETOXIFYING ENZYMES; GERMAN-COCKROACH; RESISTANCE; MOTH; MONOOXYGENASE; PURIFICATION; TEMPERATURE; LEPIDOPTERA; ISOENZYMES; EXPRESSION;
Keywords:
glutathione S-transferases; inhibitors; insecticides; insects; organophosphates; Tenebrio molitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Papadopoulos, AI Aristotelian Univ Salonika, Sch Biol, Dept Zool, Physiol Anim Lab, Thessaloniki 54006, Greece Aristotelian Univ Salonika Thessaloniki Greece 54006 ce
Citazione:
I. Kostaropoulos et al., "The role of glutathione S-transferases in the detoxification of some organophosphorus insecticides in larvae and pupae of the yellow mealworm, Tenebrio molitor (Coleoptera : Tenebrionidae)", PEST MAN SC, 57(6), 2001, pp. 501-508

Abstract

The correlation between the natural levels of glutathione S-transferase (GST) and the tolerance to the organophosphorus insecticides parathion-methyland paraoxon-methyl, as well as the interaction of affinity-purified enzyme and the insecticides were investigated in order to collect further information on the role of the glutathione S-transferase system as a mechanism ofdefence against insecticides in insects. The studies were carried out on the larvae and pupae of the coleopteran Tenebrio molitor L, which exhibit varying natural levels of GST activity. Stage-dependent susceptibility of the insect against insecticides was observed during the first 24h. However, 48h after treatment, the KD50 value increased significantly due to the recovery of some individuals. Simultaneous injection of insecticide with compounds which inhibit GST activity in vitrocaused an alteration in susceptibility of insects 24 or 48h post-treatment, depending on stage and insecticide used. Inhibition studies combined withcompetitive fluorescence spectroscopy revealed that the insecticides probably bind to the active site of the enzyme, thus inhibiting its activity towards 1-chloro-2,4-dinitrobenzene in a competitive manner. High-performance liquid chromatography and gas chromatography revealed that T molitor GST catalyses the conjugation of the insecticides studied to a reduced form of glutathione (GSH). From the above experimental results, it is considered thatGST offers a protection against the organophosphorus insecticides studied by active site binding and subsequent conjugation with GSH. (C) 2001 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:55:52