Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Establishment of an oral squamous cell carcinoma cell line (NOS-1) exhibiting amplification of the erbB-1 oncogene and point mutation of p53 tumor suppressor gene: its biological characteristics and animal model of local invasion by orthotopic transplantation of the cell line
Autore:
Ji, ZW; Oku, N; Umeda, M; Komori, T;
Indirizzi:
Kobe Univ, Sch Med, Dept Oral & Maxillofacial Surg, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 g, Chuo Ku, Kobe, Hyogo 6500017, Japan
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 4, volume: 37, anno: 2001,
pagine: 386 - 392
SICI:
1368-8375(200106)37:4<386:EOAOSC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; C-MYC; LUNG-CANCER; HEAD; NECK; ABNORMALITIES; EXPRESSION;
Keywords:
human squamous cell carcinoma cell line; erbB-1 oncogene; p53 tumor suppressor gene; mouth neoplasms; xenograft tumors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Oku, N Kobe Univ, Sch Med, Dept Oral & Maxillofacial Surg, Chuo Ku, 7-5-1 Kusunoki Cho, Kobe, Hyogo 6500017, Japan Kobe Univ 7-5-1 Kusunoki Cho Kobe Hyogo Japan 6500017 500017, Japan
Citazione:
Z.W. Ji et al., "Establishment of an oral squamous cell carcinoma cell line (NOS-1) exhibiting amplification of the erbB-1 oncogene and point mutation of p53 tumor suppressor gene: its biological characteristics and animal model of local invasion by orthotopic transplantation of the cell line", ORAL ONCOL, 37(4), 2001, pp. 386-392

Abstract

We established a new cancer cell line, NOS-I, which was derived from a human oral primary squamous cell carcinoma. Geneticin treatment was adopted toeliminate contaminating fibroblasts and to enrich tumor cells in the earlystage of the culture. The NOS-I cells showed epithelial morphological features with light and electron microscopy, and immunohistochemical analysis confirmed their epithelial origin. Overexpression of mutant p53 protein, a p53 point mutation at codon 248 with transition from CGG to TGG, and amplification of the erbB-1 oncogene/epidermal growth factor receptor gene were also observed in NOS-1 cells. The NOS-1 cells formed tumors in nude mice whentransplanted subcutaneously into their backs. Further, they were transplantable orthotopically in the tongues of nude mice, and the transplanted tumors in the tongue show-ed diffuse invasion without forming capsules. The NOS-1 cells were useful for elucidating the mechanism involving p53 inactivation and erbB-1 oncogene amplification, as well as treatment of oral cancer. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:01:21