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Titolo:
Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity
Autore:
Okamura, H; Morimoto, H; Haneji, T;
Indirizzi:
Univ Tokushima, Sch Dent, Dept Histol & Oral Histol, Tokushima 7708504, Japan Univ Tokushima Tokushima Japan 7708504 Histol, Tokushima 7708504, Japan Kyushu Dent Coll, Dept Oral Anat 1, Kitakyushu, Fukuoka 8038580, Japan Kyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 Fukuoka 8038580, Japan
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 4, volume: 37, anno: 2001,
pagine: 379 - 385
SICI:
1368-8375(200106)37:4<379:PAIOSC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER-THERAPY; DNA; INDUCTION; DEATH; LINES; MECHANISMS;
Keywords:
apoptosis; bleomycin; peplomycin; SCC cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Haneji, T Univ Tokushima, Sch Dent, Dept Histol & Oral Histol, 3-18-15 3 Kuramoto, Tokushima 7708504, Japan Univ Tokushima 3-18-15 3 Kuramoto Tokushima Japan 7708504 Japan
Citazione:
H. Okamura et al., "Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity", ORAL ONCOL, 37(4), 2001, pp. 379-385

Abstract

Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamouscarcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 muM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 muM peplomycin. How ever, SCCTF cells treated with 1 muM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5and 50 muM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:33:31