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Titolo:
Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo
Autore:
Bronk, P; Wenniger, JJ; Dawson-Scully, K; Guo, XF; Hong, S; Atwood, HL; Zinsmaier, KE;
Indirizzi:
Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Neurosci, Philadelphia, PA 19104 USA Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 ysiol, Toronto, ON M5S 1A8, Canada
Titolo Testata:
NEURON
fascicolo: 2, volume: 30, anno: 2001,
pagine: 475 - 488
SICI:
0896-6273(200105)30:2<475:DHICFN>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE-STRING PROTEIN; FROG NEUROMUSCULAR-JUNCTION; SYNAPTIC TRANSMISSION; HEAT-SHOCK; CALCIUM SENSITIVITY; TRANSMITTER RELEASE; COATED VESICLES; MELANOGASTER; CLATHRIN; CHANNEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Zinsmaier, KE Univ Penn, Sch Med, Dept Neurosci, 234D Stemmler Hall, Philadelphia, PA 19104 USA Univ Penn 234D Stemmler Hall Philadelphia PA USA 19104 4 USA
Citazione:
P. Bronk et al., "Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo", NEURON, 30(2), 2001, pp. 475-488

Abstract

Previous in vitro studies of cysteine-string protein (CSP) imply a potential role for the clathrin-uncoating ATPase Hsc70 in exocytosis. We show thathypomorphic mutations in Drosophila Hsc70-4 (Hsc4) impair nerve-evoked neurotransmitter release, but not synaptic vesicle recycling in vivo. The lossof release can be restored by increasing external or internal Ca2+ and is caused by a reduced Ca2+ sensitivity of exocytosis downstream of Ca2+ entry. Hsc4 and CSP are likely to act in common pathways, as indicated by their in vitro protein interaction, the similar loss of evoked release in individual and double mutants, and genetic interactions causing a loss of release in trans-heterozygous hsc4-csp double mutants. We suggest that Hsc4 and CSPcooperatively augment the probability of release by increasing the Ca2+ sensitivity of vesicle fusion.

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Documento generato il 06/04/20 alle ore 05:43:28