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Titolo:
Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin Bcomplex
Autore:
Mikkelsen, NE; Johansson, K; Virtanen, A; Kirsebom, LA;
Indirizzi:
Univ Uppsala, Ctr Biomed, Dept Cell & Mol Biol, SE-75124 Uppsala, Sweden Univ Uppsala Uppsala Sweden SE-75124 Mol Biol, SE-75124 Uppsala, Sweden Swedish Univ Agr Sci, Ctr Biomed, Dept Mol Biol, SE-75124 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden SE-75124 l, SE-75124 Uppsala, Sweden
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 6, volume: 8, anno: 2001,
pagine: 510 - 514
SICI:
1072-8368(200106)8:6<510:ABDADM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENYLALANINE TRANSFER-RNA; CRYSTAL-STRUCTURE; P RNA; ANTIBIOTIC INHIBITION; HAMMERHEAD RIBOZYME; ANGSTROM RESOLUTION; CLEAVAGE; RECOGNITION; REFINEMENT; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kirsebom, LA Univ Uppsala, Ctr Biomed, Dept Cell & Mol Biol, Box 596, SE-75124 Uppsala,Sweden Univ Uppsala Box 596 Uppsala Sweden SE-75124 Uppsala,Sweden
Citazione:
N.E. Mikkelsen et al., "Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin Bcomplex", NAT ST BIOL, 8(6), 2001, pp. 510-514

Abstract

Aminoglycosides bind to RNA and interfere with its function, and it has been suggested that aminoglycoside binding to RNA displaces essential divalent metal ions. Here we demonstrate that addition of various aminoglycosides inhibited Pb2+-induced cleavage of yeast tRNA(Phe), Cocrystallization of yeast tRNA(Phe) and an aminoglycoside, neomycin B, resulted in crystals that diffracted to 2.6 Angstrom and the structure of the complex was solved by molecular replacement, The structure shows that the neomycin B binding site overlaps with known divalent metal ion binding sites in yeast tRNA(Phe), providing direct evidence for the hypothesis that aminoglycosides displace metal ions. Additionally, the neomycin B binding site overlaps with major determinants for Escherichia coli phenylalanyl-tRNA-synthetase, Here we present data demonstrating that addition of neomycin B inhibited aminoacylation of E. coli tRNA(Phe) in the mid muM range. Given that aminoglycoside and metal ion binding sites overlap, we discuss that aminoglycosides can be considered as 'metal mimics'.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:09:52