Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Development of a FLP/frt system for generating helper-dependent adenoviralvectors
Autore:
Ng, P; Beauchamp, C; Evelegh, C; Parks, R; Graham, FL;
Indirizzi:
McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada McMaster Univ Hamilton ON Canada L8S 4K1 ol, Hamilton, ON L8S 4K1, Canada McMaster Univ, Dept Pathol, Hamilton, ON L8S 4K1, Canada McMaster Univ Hamilton ON Canada L8S 4K1 ol, Hamilton, ON L8S 4K1, Canada
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 3, anno: 2001,
parte:, 1
pagine: 809 - 815
SICI:
1525-0016(200105)3:5<809:DOAFSF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; IN-VIVO; IMMUNE-RESPONSES; ANTIGENS; THERAPY; CELLS; CRE; RECOMBINATION; ELIMINATION; TOXICITY;
Keywords:
adenovirus; helper-dependent adenoviral vector; helper virus; FLP recombinase; Cre recombinase; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Graham, FL McMaster Univ, Dept Biol, 1280 Main St W, Hamilton, ON L8S 4K1,Canada McMaster Univ 1280 Main St W Hamilton ON Canada L8S 4K1 Canada
Citazione:
P. Ng et al., "Development of a FLP/frt system for generating helper-dependent adenoviralvectors", MOL THER, 3(5), 2001, pp. 809-815

Abstract

Helper-dependent (HD) adenoviral vectors devoid of all viral coding sequences have a large cloning capacity and have been reported to provide long-term transgene expression in vivo with negligible toxicity, making them attractive vectors for gene therapy. Currently, the most efficient means of generating HD vectors involves co-infecting 293 cells expressing Cre with the HD vector and a helper virus bearing a packaging signal flanked by IoxP sites. Cre-mediated excision of the packaging signal renders the helper virus genome unpackageable but still able to replicate and to provide helper functions for HD vector propagation. HD vector titer is increased by serial coinfections. Typically helper virus contamination is less than or equal to1% pre- and less than or equal to0.1% postpurification by CsCI banding. While these contamination levels are low, further reduction is desirable. Alternative methods of selection against the helper virus may achieve this goal, especially when combined with Cre/IoxP. We describe the development of a system for generating HD vectors based on site-specific recombination between frt sites catalyzed by FLP recombinase and show by direct comparison that the FLP/frt and Cre/IoxP systems are equivalent with respect to HD vector amplification efficiency and helper virus contamination levels. Availability of a second recombinase system for HD vector production will enhance the utility and flexibility of NO vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:35:17