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Titolo:
A novel minimal-size vector (MIDGE) improves transgene expression in coloncarcinoma cells and avoids transfection of undesired DNA
Autore:
Schakowski, F; Gorschluter, M; Junghans, C; Schroff, M; Buttgereit, P; Ziske, C; Schottker, B; Konig-Merediz, SA; Sauerbruch, T; Wittig, B; Schmidt-Wolf, IGH;
Indirizzi:
Univ Bonn, Dept Internal Med 1, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 ept Internal Med 1, D-53105 Bonn, Germany Mologen GmbH, D-14195 Berlin, Germany Mologen GmbH Berlin Germany D-14195 ologen GmbH, D-14195 Berlin, Germany Free Univ Berlin, Klinikum Benjamin Franklin, Inst Mol Biol & Biochem, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 Biochem, D-14195 Berlin, Germany
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 3, anno: 2001,
parte:, 1
pagine: 793 - 800
SICI:
1525-0016(200105)3:5<793:ANMV(I>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSFER; NUCLEOCYTOPLASMIC TRANSPORT; IN-VIVO; THERAPY; DELIVERY; CANCER; SEQUENCES;
Keywords:
MIDGE vector; plasmid; nonviral gene transfer; transfection efficiency; colon carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt-Wolf, IGH Univ Bonn, Dept Internal Med 1, Sigmund Freud Str 25, D-53105 Bonn, Germany Univ Bonn Sigmund Freud Str 25 Bonn Germany D-53105 any
Citazione:
F. Schakowski et al., "A novel minimal-size vector (MIDGE) improves transgene expression in coloncarcinoma cells and avoids transfection of undesired DNA", MOL THER, 3(5), 2001, pp. 793-800

Abstract

Viral and plasmid vectors may cause unwanted immunological side effects resulting from the expression of nontherapeutic genes contained in their sequence. Furthermore, replication-defective viral vectors carry the potential risk of recombination with wild-type viruses or activation of oncogenes. A new vector type for minimalistic, immunologically defined gene expression (MIDGE) may overcome these problems. MIDGE is a minimal-size gene transfer unit containing the expression cassette, including promoter, gene, and RNA-stabilizing sequence, flanked by two short hairpin oligonucleotide sequences. The resulting vector is a small, linear, covalently closed, dumb-bell-shaped molecule. DNA not encoding the desired gene is reduced to a minimum. Here, we transfected colon carcinoma cell lines using cationic lipid, cationic polymer, and electroporation with several MIDGE vectors and correspondingplasmids containing transgenes encoding enhanced green fluorescent protein(eGFP) and human interleukin-2 (hIL-2). Transfection efficiency as measured qualitatively and quantitatively with eGFP was found to be comparable forboth vector types. However, hIL-2 secretion and eGFP expression were approximately two- to fourfold higher in most cells transfected with these transgenes using MIDGE vectors compared to the plasmid control. This report demonstrates the advantages of this new vector type and its prospects for ex vivo gene therapy studies.

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Documento generato il 20/02/20 alle ore 03:36:03