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Titolo:
Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach
Autore:
Zhang, XW; Li, CY;
Indirizzi:
Duke Univ, Med Ctr, Dept Radiat Oncol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 tr, Dept Radiat Oncol, Durham, NC 27710 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 3, anno: 2001,
parte:, 1
pagine: 787 - 792
SICI:
1525-0016(200105)3:5<787:GORAVV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOASSOCIATED VIRUS; GENE-TRANSFER; AAV VECTOR; IMMUNOCOMPETENT MICE; CELL-LINE; FACTOR-IX; EXPRESSION; TITER; HELPER; MUSCLE;
Keywords:
adeno-associated virus production; adenovirus; inducible rep expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Li, CY Duke Univ, Med Ctr, Dept Radiat Oncol, Box 3455, Durham, NC 27710 USA Duke Univ Box 3455 Durham NC USA 27710 x 3455, Durham, NC 27710 USA
Citazione:
X.W. Zhang e C.Y. Li, "Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach", MOL THER, 3(5), 2001, pp. 787-792

Abstract

An efficient approach to the large-scale production of rAAV will significantly facilitate the application of this promising gene delivery vector in human gene therapy applications. In this study, we report a novel approach to rAAV production that is based exclusively on the adenovirus vector, without the need for plasmid transfections and special packaging cell lines. Allcomponents required for rAAV production, including the rep and cop genes, and the therapeutic gene(s) are delivered to the widely used 293 packaging cell line by adenovirus vectors. High-titer rAAV vectors (200-600 infectious units/producer cell) were obtained by use of this approach. As adenovirusvectors can be produced to high titers and they can infect cells in suspension efficiently, this approach may be amenable to scaled-up production of rAAV vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 23:40:02