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Titolo:
HSV-1 vector-delivered FGF2 to the retina is neuroprotective but does not preserve functional responses
Autore:
Spencer, B; Agarwala, S; Gentry, L; Brandt, CR;
Indirizzi:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 biol & Immunol, Madison, WI 53706 USA Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 l & Visual Sci, Madison, WI 53706 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 3, anno: 2001,
parte:, 1
pagine: 746 - 756
SICI:
1525-0016(200105)3:5<746:HVFTTR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; SIMPLEX VIRUS TYPE-1; RIBONUCLEOTIDE REDUCTASE; LIGHT DAMAGE; PHOTORECEPTOR DEGENERATION; NEUROTROPHIC FACTOR; FISCHER-344 RATS; CONSTANT LIGHT; ALBINO-RATS; OPTIC-NERVE;
Keywords:
neuroprotection; FCF2; bFGF; HSV vectors; retinal degeneration; electroretinogram; PC12 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Brandt, CR 6630 Med Sci Ctr,1300 Univ Ave, Madison, WI 53706 USA 6630 MedSci Ctr,1300 Univ Ave Madison WI USA 53706 53706 USA
Citazione:
B. Spencer et al., "HSV-1 vector-delivered FGF2 to the retina is neuroprotective but does not preserve functional responses", MOL THER, 3(5), 2001, pp. 746-756

Abstract

Fibroblast growth factor 2 (bFGF, FGF2) exhibits mitogenic, angiogenic, wound healing, and neuroprotective properties. Infusion of FGF2 in vivo to treat neurodegenerative disorders in animal models results in increased survival of damaged neurons, but these effects are transient. To test the feasibility of HSV vector-delivered FGF2 for neuroprotection, we inserted the FGF2 gene under the control of the HCMV immediate-early promoter into an attenuated avirulent HSV-1 vector. Transduction with FGF2/HSV-1 virus promoted survival of PC12 cells, induced differentiation of these cells to the neuronal phenotype in vitro, and protected PC12 neuronal cells from death inducedby nerve growth factor withdrawal. The attenuated FGF2/HSV-1 virus was able to deliver and direct expression of the FGF2 gene in the eye. Delivery prior to light exposure in a rat model of retinal degeneration resulted in significant protection against photoreceptor loss. However, functional ERG responses were not detected. Treatment of normal eyes with the vector alone suppressed ERGs, which were only partially restored in eyes receiving the FGF2 vector. Thus, although the FGF2-HSV-1 virus induced preservation of celland tissue structure, this was not sufficient to protect photoreceptor function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:36:21