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Titolo:
Use of perfluorochemical liquid allows earlier detection of gene expression and use of less vector in normal lung and enhances gene expression in acutely injured lung
Autore:
Weiss, DJ; Bonneau, L; Liggitt, D;
Indirizzi:
Fred Hutchinson Canc Res Ctr, Div Pulm & Crit Care Med, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Univ Washington, Sch Med, Dept Comparat Med, Seattle, WA 98105 USA Univ Washington Seattle WA USA 98105 Comparat Med, Seattle, WA 98105 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 3, anno: 2001,
parte:, 1
pagine: 734 - 745
SICI:
1525-0016(200105)3:5<734:UOPLAE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMEMBRANE CONDUCTANCE REGULATOR; ADENOVIRUS-MEDIATED TRANSFER; CYSTIC-FIBROSIS; ALVEOLAR MACROPHAGES; BETA-GALACTOSIDASE; NONHUMAN-PRIMATES; IN-VITRO; RESPIRATORY-TRACT; AIRWAY EPITHELIA; SENSITIVE METHOD;
Keywords:
gene therapy; lung; perfluorochemical liquid; adenovirus; acute lung injury; bleomycin; beta-galactosidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Weiss, DJ Fred Hutchinson Canc Res Ctr, Div Pulm & Crit Care Med, MailstopD3-190,1100 Fairview Ave N, Seattle, WA 98109 USA Fred Hutchinson Canc ResCtr Mailstop D3-190,1100 Fairview Ave N Seattle WA USA 98109
Citazione:
D.J. Weiss et al., "Use of perfluorochemical liquid allows earlier detection of gene expression and use of less vector in normal lung and enhances gene expression in acutely injured lung", MOL THER, 3(5), 2001, pp. 734-745

Abstract

One of the obstacles to successful lung gene transfer is effective delivery of vector to lung, particularly injured or diseased lung. We have previously demonstrated that intratracheal instillation of perfluorochemical (PFC)liquids along with instillation of recombinant adenovirus and adeno-associated virus vectors,or with cationic liposome vectors, increased total lung gene expression and enhanced distribution of gene expression throughout thelung. To further explore the potential benefits of PFC liquid use, we evaluated the effect of PFC liquid instillation on several other aspects of adenovirus-mediated gene expression in lung. Use of PFC liquid resulted in earlier detection of gene expression and allowed the use of less vector to achieve expression comparable to that observed with the use of higher amounts of vector alone. Using PFC liquid also enhanced gene expression in a rodentmodel of acute lung injury. PFC liquid did cause a transient inflammation when instilled into normal lungs but did not cause any additional inflammation when instilled alone or with adenovirus vector into acutely injured lungs. Thus, PFC liquid may be a useful adjunct for clinical lung gene transfer, particularly for injured or diseased lungs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:56:18