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Titolo:
C/EBP alpha and G-CSF receptor signals cooperate to induce the myeloperoxidase and neutrophil elastase genes
Autore:
Wang, W; Wang, X; Ward, AC; Touw, IP; Friedman, AD;
Indirizzi:
Johns Hopkins Oncol Ctr, Div Pediat Oncol, Baltimore, MD USA Johns HopkinsOncol Ctr Baltimore MD USA Pediat Oncol, Baltimore, MD USA Erasmus Univ, Inst Hematol, Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands Inst Hematol, Rotterdam, Netherlands
Titolo Testata:
LEUKEMIA
fascicolo: 5, volume: 15, anno: 2001,
pagine: 779 - 786
SICI:
0887-6924(200105)15:5<779:CAAGRS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING-FACTOR; BINDING PROTEIN-ALPHA; PROGENITOR-CELL LINE; DEFICIENT MICE; GRANULOCYTIC DIFFERENTIATION; TRANSCRIPTION FACTOR; MYELOID CELLS; INCREASED APOPTOSIS; PU.1 SPI-1; 32D CELLS;
Keywords:
C/EBP alpha; G-CSF receptor; granulopoiesis; hematopoiesis; cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Friedman, AD Johns Hopkins Univ, Canc Res Bldg,Room 255,1650 Orleans St, Baltimore, MD 21231 USA Johns Hopkins Univ Canc Res Bldg,Room 255,1650 Orleans St Baltimore MD USA 21231
Citazione:
W. Wang et al., "C/EBP alpha and G-CSF receptor signals cooperate to induce the myeloperoxidase and neutrophil elastase genes", LEUKEMIA, 15(5), 2001, pp. 779-786

Abstract

'To assess cooperation between G-CSF signals and C/EBP alpha, we characterized Ba/F3 pro-B cell lines expressing C/EBP alpha WT-ER and the G-CSF receptor (GCSFR). In these lines, GCSFR signals can be evaluated independent oftheir effect on C/EBP alpha levels. G-CSF alone did not induce the MPG, NE, LF, or PU.1 RNAs, and C/EBP alpha WT-ER alone stimulated low-level MPO and high-level PU.1 expression. Simultaneous activation of the GCSFR and C/EBP alpha WT-ER markedly increased MPO and NE induction at 24 h, and LF mRNA was detected at 48 h. G-CSF did not increase endogenous GCSFR, endogenous C/EBP alpha or exogenous C/EBP alpha WT-ER levels, and C/EBP alpha WT-ER didnot induce endogenous or exogenous GCSFR. Several GCSFR mutants were also co-expressed with C/EBP alpha WT-ER. Mutation of all four cytoplasmic tyrosines prevented NE induction but enhanced MPO induction. Mutation of Y704 was required for increased MPO induction. Consistent with this finding, removing IL-3 without G-CSF addition enabled MPG, but not NE, induction by C/EBPalpha WT-ER. GCSFR signals or related signals from other receptors may cooperate with C/EBP alpha to direct differentiation of normal myeloid stem cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 00:00:58