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Titolo:
Postentry restriction to human immunodeficiency virus-based vector transduction in human monocytes
Autore:
Neil, S; Martin, F; Ikeda, Y; Collins, M;
Indirizzi:
Univ Coll London, Windeyer Inst Med Sci, Dept Immunol & Mol Pathol, LondonW1P 6DB, England Univ Coll London London England W1P 6DB l Pathol, LondonW1P 6DB, England
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 12, volume: 75, anno: 2001,
pagine: 5448 - 5456
SICI:
0022-538X(200106)75:12<5448:PRTHIV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYPE-1 REVERSE TRANSCRIPTION; DENDRITIC CELLS; IN-VITRO; T-CELLS; PRIMARY MACROPHAGES; LENTIVIRAL VECTOR; PRODUCTIVE INFECTION; NONDIVIDING CELLS; NUCLEAR IMPORT; SIGNAL-TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Collins, M Univ Coll London, Windeyer Inst Med Sci, Dept Immunol & Mol Pathol, 46 Cleveland St, London W1P 6DB, England Univ Coll London 46 ClevelandSt London England W1P 6DB ngland
Citazione:
S. Neil et al., "Postentry restriction to human immunodeficiency virus-based vector transduction in human monocytes", J VIROLOGY, 75(12), 2001, pp. 5448-5456

Abstract

Cells of the monocyte lineage can be infected with human immunodeficiency virus type 1 (HIV-1) both during clinical infection and in vitro. The ability of HIV-l-based vectors to transduce human monocytes, monocyte-derived macrophages, and dendritic cells (DCs) was therefore examined, in order to develop an efficient protocol for antigen gene delivery to human antigen-presenting cells. Freshly isolated monocytes were refractory to HIV-1-based vector transduction but became transducible after in vitro differentiation to mature macrophages. This maturation-dependent transduction was independent of the HIV-1 accessory proteins Vif, Vpr, Vpu, and Nef in the packaging cells and of the central polypurine tract in the vector, and it was also observed with a vesicular stomatitis virus-pseudotyped HIV-1 provirus, defectiveonly in envelope and Nef, The level and extent of reverse transcription ofthe HIV-1-based vector was similar after infection of immature monocytes and of mature macrophages. However, 2LTR vector circles could not be detected in monocytes, suggesting a block to vector nuclear entry in these cells. Transduction of freshly isolated monocytes exposed to HIV-1-based vector could be rescued by subsequent differentiation into DCs, This rescue was induced by fetal calf serum in the DC culture medium, which promoted vector nuclear entry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 08:58:20