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Titolo:
Herpes simplex virus nucleocapsids mature to progeny virions by an envelopment -> deenvelopment -> reenvelopment pathway
Autore:
Skepper, JN; Whiteley, A; Browne, H; Minson, A;
Indirizzi:
Univ Cambridge, Dept Pathol, Div Virol, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP rol, Cambridge CB2 1QP, England Univ Cambridge, Multi Imaging Ctr, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP Ctr, Cambridge CB2 1QP, England Univ Cambridge, Dept Anat, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP nat, Cambridge CB2 1QP, England
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 12, volume: 75, anno: 2001,
pagine: 5697 - 5702
SICI:
0022-538X(200106)75:12<5697:HSVNMT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; VARICELLA-ZOSTER VIRUS; ENDOPLASMIC-RETICULUM; ANTEROGRADE TRANSPORT; PSEUDORABIES VIRUS; EGRESS; TYPE-1; GLYCOPROTEINS; GENE; MICROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Minson, A Univ Cambridge, Dept Pathol, Div Virol, Tennis Court Rd, Cambridge CB2 1QP, England Univ Cambridge Tennis Court Rd Cambridge England CB2 1QP ngland
Citazione:
J.N. Skepper et al., "Herpes simplex virus nucleocapsids mature to progeny virions by an envelopment -> deenvelopment -> reenvelopment pathway", J VIROLOGY, 75(12), 2001, pp. 5697-5702

Abstract

Herpes simplex virus (HSV) nucleocapsids acquire an envelope by budding through the inner nuclear membrane, but it is uncertain whether this envelopeis retained during virus maturation and egress or whether mature progeny virions are derived by deenvelopment at the outer nuclear membrane followed by reenvelopment in a cytoplasmic compartment, To resolve this issue, we used immunogold electron microscopy to examine the distribution of glycoprotein D (gD) in cells infected with HSV-1 encoding a wild-type go or a go which is retrieved to the endoplasmic reticulum (ER). In cells infected with wild-type HSV-1, extracellular virions and virions in the perinuclear space bound approximately equal amounts of go antibody. In cells infected with HSV-I encoding an ER-retrieved go, the inner and outer nuclear membranes were heavily gold labeled, as were perinuclear enveloped virions. Extracellular virions exhibited very little gold decoration (10- to 30-fold less than perinuclear virions). We conclude that the envelope of perinuclear virions must be lost during maturation and egress and that mature progeny virions mustacquire an envelope from a post-ER cytoplasmic compartment, We noted also that go appears to be excluded from the plasma membrane in cells infected with wild-type virus.

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Documento generato il 29/11/20 alle ore 00:40:52