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Titolo:
The use of Ag85 complex as antigen in ELISA for the diagnosis of bovine tuberculosis in dairy cows in Brazil
Autore:
Lilenbaum, W; Pessolani, MCV; Fonseca, LS;
Indirizzi:
Univ Fed Fluminense, Dept Microbiol, BR-24210130 Rio De Janeiro, Brazil Univ Fed Fluminense Rio De Janeiro Brazil BR-24210130 BC Janeiro, Brazil
Titolo Testata:
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARYPUBLIC HEALTH
fascicolo: 3, volume: 48, anno: 2001,
pagine: 161 - 166
SICI:
0931-1793(200104)48:3<161:TUOACA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; MYCOBACTERIUM-BOVIS; PROTEIN; CATTLE; SERODIAGNOSIS; PURIFICATION; ANTIBODIES; RESPONSES; PPD; BCG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Lilenbaum, W Univ Fed Fluminense, Dept Microbiol, R Prof Hernani Mello 101, BR-24210130Rio De Janeiro, Brazil Univ Fed Fluminense R Prof Hernani Mello 101 Rio De Janeiro Brazil BR-24210130 BC
Citazione:
W. Lilenbaum et al., "The use of Ag85 complex as antigen in ELISA for the diagnosis of bovine tuberculosis in dairy cows in Brazil", J VET MED B, 48(3), 2001, pp. 161-166

Abstract

Several enzyme-linked immunosorbent assay (ELISA) methods have been investigated to evaluate their performance in the diagnosis of tuberculosis in cattle. Increased production of antibodies to the proteins of antigen 85 complex (Ag85) after experimental and natural infection in cattle shows that they are strongly immunogenic in vivo. The purpose of this study was to evaluate the use of Ag85 as an antigen in ELISA for the diagnosis of bovine tuberculosis in dairy cows in Brazil. The test groups consisted of 46 serum samples from intradermal tuberculin test (ITT)-positive animals (Group A) and 46 samples from ITT-negative animals (Group B). Group C comprised 12 samples from a tuberculosis-free herd and was the control group of the study. Samples were tested in an ELISA using Ag85 as antigen. Differences between themean ODs of groups A and B and A and C were significant (P < 0.01), but nosignificant difference (P > 0.05) was observed between groups B and C. Thesensitivity of the ELISA using Ag85 was 91.3 % (42/46) and its specificitywas 94.8 % (55/58). These results were not significantly different (P > 0.05) from those observed in a previous study of an ELISA using purified protein derivative (PPD). We concluded that, although Ag85 can be used as antigen for ELISA tests in the diagnosis of bovine tuberculosis with good sensitivity and specificity rates, no significant advantages were observed in relation to the ELISA using PPD that could justify the purification and utilization of Ag85 as a single antigen in routine methods of diagnosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:27:33