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Titolo:
Synapse-glia interactions at the mammalian neuromuscular junction
Autore:
Rochon, D; Rousse, I; Robitaille, R;
Indirizzi:
Univ Montreal, Ctr Rech Sci Neurol, Dept Physiol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 11, volume: 21, anno: 2001,
pagine: 3819 - 3829
SICI:
0270-6474(20010601)21:11<3819:SIATMN>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERISYNAPTIC SCHWANN-CELLS; DEPENDENT CALCIUM CHANNELS; ASTROCYTES IN-SITU; INTRACELLULAR CALCIUM; ADENINE-NUCLEOTIDES; NERVE-TERMINALS; ATP; TRANSMITTER; MODULATION; INCREASES;
Keywords:
adenosine; acetylcholine; Ca2+; perisynaptic Schwann cell; transmitter release; neuromuscular junction; synapse-glia interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Robitaille, R Univ Montreal, Ctr Rech Sci Neurol, Dept Physiol, POB 6128,Stn Ctrville, Montreal, PQ H3C 3J7, Canada Univ Montreal POB 6128,Stn Ctrville Montreal PQ Canada H3C 3J7
Citazione:
D. Rochon et al., "Synapse-glia interactions at the mammalian neuromuscular junction", J NEUROSC, 21(11), 2001, pp. 3819-3829

Abstract

Perisynaptic Schwann cells (PSCs) play critical roles in regulating and stabilizing nerve terminals at the mammalian neuromuscular junction (NMJ). However, although these functions are likely regulated by the synaptic properties, the interactions of PSCs with the synaptic elements are not known. Therefore, our goal was to study the interactions between mammalian PSCs in situ and the presynaptic terminals using changes in intracellular Ca2+ as anindicator of cell activity. Motor nerve stimulation induced an increase inintracellular Ca2+ in PSCs, and this increase yas greatly reduced when transmitter release was blocked. Furthermore, local application of acetylcholine induced Ca2+ responses that were blocked by the muscarinic antagonist atropine and mimicked by the muscarinic agonist muscarine. The nicotinic antagonist alpha -bungarotoxin had no effect on Ca2+ responses induced by acetylcholine. Local application of the cotransmitter ATP induced Ca2+ responsesthat were unaffected by the P2 antagonist suramin, whereas local application of adenosine induced Ca2+ responses that were greatly reduced by the A1 receptor antagonist 8-cyclopentyl-1,3-dimethylxanthine (CPT). However, the presence of the A1 antagonist in the perfusate did not block responses induced by ATP. Ca2+ responses evoked by stimulation of the motor nerve were reduced in the presence of CPT, whereas atropine almost completely abolished them. Ca2+ responses were further reduced when both antagonists were present simultaneously. Hence, PSCs at the mammalian NMJ respond to the release of neurotransmitter induced by stimulation of the motor nerve through the activation of muscarinic and adenosine A1 receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:17:55