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Titolo:
Contraction-dependent apoptosis of normal dermal fibroblasts
Autore:
Niland, S; Cremer, A; Fluck, J; Eble, JA; Krieg, T; Sollberg, S;
Indirizzi:
Univ Cologne, Dept Dermatol, D-50924 Cologne, Germany Univ Cologne Cologne Germany D-50924 Dermatol, D-50924 Cologne, Germany Univ Munster, Inst Physiol Chem, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 hysiol Chem, D-4400 Munster, Germany
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 5, volume: 116, anno: 2001,
pagine: 686 - 692
SICI:
0022-202X(200105)116:5<686:CAONDF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH MUSCLE ACTIN; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; CELL-SURVIVAL; COLLAGEN LATTICES; INTEGRINS; ADHESION; BINDING; GROWTH; TISSUE;
Keywords:
apoptosis; cell-matrix interaction; cytochalasin D; fibroblastic cell; integrin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Niland, S Univ Cologne, Dept Dermatol, D-50924 Cologne, Germany Univ Cologne Cologne Germany D-50924 D-50924 Cologne, Germany
Citazione:
S. Niland et al., "Contraction-dependent apoptosis of normal dermal fibroblasts", J INVES DER, 116(5), 2001, pp. 686-692

Abstract

The mechanisms underlying the contraction-dependent apoptosis of primary fibroblasts are of prime importance in understanding anchorage-dependent survival/apoptosis of dermal fibroblasts. As integrins are essential extracellular matrix receptors in fibroblasts, their role in anchorage-dependent apoptosis/ survival of fibroblasts was analyzed. Primary human fibroblasts displayed a marked reduction of apoptosis in mechanically relaxed collagen matrices in the presence of adhesion-blocking antibodies against alpha (1)beta(1) or alpha (2)beta (1). Anti-alpha (v)beta (3) antibodies had a considerably weaker effect. In additional experiments RD cells, which lack alpha (2) integrin, displayed no apoptosis in mechanically relaxed collagen matrices. Their susceptibility to apoptosis was restored after transfection with functional alpha (2) integrin, and it could be blocked again by adhesion-blocking antibodies against alpha (2)beta (1) integrin. Therefore we conclude that apoptosis of human primary fibroblasts in contractile collagen matrices is - at least in part - inhibited by adhesion-blocking anti-integrin antibodies, suggesting that the mode of apoptosis in this case is different from anoikis. Further, apoptosis in a mechanically relaxed collagen matrix could be abrogated by depolymerization of F-actin using cytochalasin D and also by disturbing actin-myosin interaction using 2,3-butanedione monoxime, indicating a possible dependence of apoptosis on mechanical forces and/or cell shape.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 23:54:40