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Titolo:
Epstein-Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1 delta (EF-1 delta): EF-1 delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells
Autore:
Kato, K; Kawaguchi, Y; Tanaka, M; Igarashi, M; Yokoyama, A; Matsuda, G; Kanamori, M; Nakajima, K; Nishimura, Y; Shimojima, M; Phung, HTT; Takahashi, E; Hirai, K;
Indirizzi:
Tokyo Med & Dent Univ, Dept Tumor Virol, Div Virol & Immunol, Med Res Inst,Bunkyo Ku, Tokyo 1138510, Japan Tokyo Med & Dent Univ Tokyo Japan 1138510 unkyo Ku, Tokyo 1138510, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Microbiol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 icrobiol, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 6
pagine: 1457 - 1463
SICI:
0022-1317(200106)82:<1457:EVPKBM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARICELLA-ZOSTER VIRUS; HERPES-SIMPLEX VIRUS; HUMAN CYTOMEGALOVIRUS; INFECTED-CELLS; U(L)13 GENE; PHOSPHORYLATION; GANCICLOVIR; GAMMA; ICP0; PHOSPHOTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kawaguchi, Y Tokyo Med & Dent Univ, Dept Tumor Virol, Div Virol & Immunol,Med Res Inst,Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138510 pan
Citazione:
K. Kato et al., "Epstein-Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1 delta (EF-1 delta): EF-1 delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells", J GEN VIROL, 82, 2001, pp. 1457-1463

Abstract

Translation elongation factor 1 delta (EF-1 delta) is hyperphosphorylated in various mammalian cells infected with alpha-, beta- and gammaherpesviruses and EF-1 delta modification is mediated by viral protein kinases, including UL13 of herpes simplex virus type 1 and UL97 of human cytomegalovirus. In this study, the following is reported, (i) BGLF4 encoded by the prototype gammaherpesvirus Epstein-Barr virus was purified as a fusion protein thatwas labelled with [gamma-P-32]ATP and labelling was eliminated by phosphatase. (ii) The ratio of the hyperphosphorylated form of human EF-1 delta wasincreased both in Sf9 cells after infection with baculoviruses expressing GST-BGLF4 fusion proteins and in COS-7 cells after transfection with a BGLF4 expression plasmid, These results indicate that purified BGLF4 possesses protein kinase activity and mediates EF-1 delta hyperphosphorylation. Thesedata also support the hypothesis that the protein kinases that are conserved by herpesviruses universally mediate EF-1 delta modification in mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:16:36