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Titolo:
Characterization and application of monoclonal antibodies against white spot syndrome virus
Autore:
Shih, HH; Wang, CS; Tan, LF; Chen, SN;
Indirizzi:
Natl Taiwan Univ, Dept Zool, Taipei 106, Taiwan Natl Taiwan Univ Taipei Taiwan 106 n Univ, Dept Zool, Taipei 106, Taiwan
Titolo Testata:
JOURNAL OF FISH DISEASES
fascicolo: 3, volume: 24, anno: 2001,
pagine: 143 - 150
SICI:
0140-7775(200103)24:3<143:CAAOMA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNDROME BACULOVIRUS WSBV; POLYMERASE CHAIN-REACTION; PENAEUS-MONODON; MESODERMAL BACULOVIRUS; SHRIMP; WSSV; DNA; PATHOGENICITY; INFECTION; JAPONICUS;
Keywords:
diagnosis; MAbs; Penaeus monodon; white spot syndrome virus; WSSV; IFA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Shih, HH Natl Taiwan Univ, Dept Zool, Taipei 106, Taiwan Natl Taiwan UnivTaipei Taiwan 106 ept Zool, Taipei 106, Taiwan
Citazione:
H.H. Shih et al., "Characterization and application of monoclonal antibodies against white spot syndrome virus", J FISH DIS, 24(3), 2001, pp. 143-150

Abstract

Three hybridoma clones secreting monoclonal antibodies (MAbs) were produced from mouse myeloma and spleen cells immunized with white spot syndrome virus (WSSV) isolated and purified from Penaeus monodon (Fabricius), collected from north-eastern Taiwan. By sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the protein profile of this isolate contained four major proteins with sizes of approximately 35 (VP35), 28 (VP28), 24 (VP24), and 19 kDa (VP19). Western blot analysis revealed that two MAbs (1D7 and 6E1) recognized epitopes on VP28 and one MAb (3E8) recognized an epitopeon VP19. The MAb 6E1 isotyped to the IgG(1) class was used in both an indirect immunofluorescence assay (IFA) and in an immunochemical staining protocol for successful identification and localization of WSSV in infected shrimp tissues. Antigenic similarity of isolates from Indonesia and Malaysia tothe Taiwan isolate was illustrated by IFA with MAb 6E1.A MAb (2F6) which bound specifically to two shrimp proteins, 75 and 72 kDa, and reacted to thehealthy and non-target tissues of WSSV in infected shrimp, such as hepatopancreas, is also described here and shows the necessity for specific identification of antibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/11/18 alle ore 03:34:13