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Titolo:
Role of stem cell factor and monocyte chemoattractant protein-1 in the interaction between fibroblasts and mast cells in fibrosis
Autore:
Yamamoto, T; Hartmann, K; Eckes, B; Krieg, T;
Indirizzi:
Univ Cologne, Dept Dermatol, D-5000 Cologne, Germany Univ Cologne Cologne Germany D-5000 pt Dermatol, D-5000 Cologne, Germany
Titolo Testata:
JOURNAL OF DERMATOLOGICAL SCIENCE
fascicolo: 2, volume: 26, anno: 2001,
pagine: 106 - 111
SICI:
0923-1811(200106)26:2<106:ROSCFA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; GROWTH-FACTOR; GENE-EXPRESSION; PULMONARY FIBROSIS; KIT-LIGAND; SCLERODERMA; PROMOTION; LEUKEMIA; COLLAGEN; DISEASE;
Keywords:
MCP-I; fibrosis; scleroderma; mast cell; stem cell factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, T Tokyo Med & Dent Univ, Sch Med, Dept Dermatol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138519, Japan Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138519 apan
Citazione:
T. Yamamoto et al., "Role of stem cell factor and monocyte chemoattractant protein-1 in the interaction between fibroblasts and mast cells in fibrosis", J DERMA SCI, 26(2), 2001, pp. 106-111

Abstract

Mast cell infiltration and accumulation is known to occur in tissue fibrosis. Increased numbers of mast cells are detected in scleroderma or hypertrophic scar skin, however, neither the role of mast cells nor the interactionof fibroblasts and mast cells in fibrosis are fully understood. A growing body of evidence indicate that mast cells are rich source of cytokines, growth factors or chemokines, which are suggested to play an important role inthe induction of fibrosis. Recent in vivo and in vitro studies suggest theinvolvement of monocyte chemoattractant protein-1 (MCP-1), a member of theC-C chemokine family, in fibrosis. Here, we examined the effect of stem cell factor (SCF), a mast cell growth factor, on MCP-1 gene expression in a human mast cell line, HMC-1, and as well as the effect of MCP-1 on alpha1(I)collagen gene expression in human skin fibroblasts. HMC-1 cells spontaneously expressed MCP-1 mRNA transcripts, which was detectable by in situ hybridization and Northern blot analysis. Stimulation with SCF further upregulated MCP-1 mRNA expression in a time- and dose-dependent manner, and stimulation with 100 ng/ml SCF for 24 h induced a 3-fold increase of MCP-1 mRNA expression in HMC-1 cells as compared with unstimulated cells. The concentration of MCP-1 protein in the culture supernatants of 50 ng/ml SCF-stimulated HMC-1 cells (3816 +/- 70 pg/ml) was significantly elevated compared to unstimulated cells (2588 +/- 130 pg/ml) (P < 0.01), as assessed by ELISA. Adversely, MCP-1 induced <alpha>1(I) collagen mRNA expression in normal skin fibroblasts dose-dependently. Finally, comparative study revealed that the concentration of SCF in the culture supernatants of scleroderma fibroblasts atprimary passages was significantly increased (344.6 +/- 182.4 pg/ml), as compared with normal skin fibroblasts (72.4 +/- 20.2 pg/ml) (P < 0.05). These results suggest that fibroblast-derived SCF upregulates MCP-1 expression and synthesis in mast cells, which acts on fibroblasts to enhance al(I) collagen mRNA expression. Our data may indicate an important interaction of fibroblasts and mast cells, via SCF and MCP-1, in the induction of fibrosis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:36:10