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Titolo:
Detection of trace amounts of hidden allergens: hazelnut and almond proteins in chocolate
Autore:
Scheibe, B; Weiss, W; Rueff, F; Przybilla, B; Gorg, A;
Indirizzi:
Tech Univ Munich, Inst Lebensmitteltechnol & Analyt Chem, D-8050 Freising,Germany Tech Univ Munich Freising Germany D-8050 t Chem, D-8050 Freising,Germany Univ Munich, Klin & Poliklin Dermatol & Allergol, Munich, Germany Univ Munich Munich Germany oliklin Dermatol & Allergol, Munich, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 756, anno: 2001,
pagine: 229 - 237
SICI:
1387-2273(20010525)756:1-2<229:DOTAOH>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHEAT-GRAIN ALLERGENS; IDENTIFICATION; QUANTIFICATION; POLYACRYLAMIDE; NITROCELLULOSE; ELISA; FOODS; GELS;
Keywords:
food allergy; immunoblotting; almond; hazelnut; proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Scheibe, B Tech Univ Munich, Inst Lebensmitteltechnol & Analyt Chem, D-8050 Freising,Germany Tech Univ Munich Freising Germany D-8050 050 Freising,Germany
Citazione:
B. Scheibe et al., "Detection of trace amounts of hidden allergens: hazelnut and almond proteins in chocolate", J CHROMAT B, 756(1-2), 2001, pp. 229-237

Abstract

Many patients with immediate type allergy to tree pollen also suffer from intolerance to hazelnuts and almonds. Since rather low levels of hazelnut and almond proteins can provoke an allergic reaction in sensitized individuals, an immunoblot technique has been developed for the detection of potentially allergenic hazelnut and almond proteins in chocolate. Initially, IgE binding hazelnut and almond proteins were detected by immunoprobing with allergic patients' sera. For routine analysis, patients' sera were substitutedwith polyclonal rabbit antisera, and sensitivity was enhanced by the use of a chemiluminescent detection method. This technique allowed the detectionof less than 0.5 mg of hazelnut or almond proteins per 100 g of chocolate (=5 ppm). It was applied for routine screening purposes in product quality control as well as for optimization of cleaning steps of filling facilitiesto minimize cross contamination during production. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 24/01/20 alle ore 12:56:10