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Titolo:
Simultaneous determination of the histamine H-1-receptor antagonist ebastine and its two metabolites, carebastine and hydroxyebastine, in human plasma using high-performance liquid chromatography
Autore:
Matsuda, M; Mizuki, Y; Terauchi, Y;
Indirizzi:
Dainippon Pharmaceut Co Ltd, Dev Res Labs, Dept Pharmacokinet, Osaka 5640053, Japan Dainippon Pharmaceut Co Ltd Osaka Japan 5640053 et, Osaka 5640053, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 757, anno: 2001,
pagine: 173 - 179
SICI:
1387-2273(20010605)757:1<173:SDOTHH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEALTHY-SUBJECTS; ANTIHISTAMINE;
Keywords:
ebastine; carebastine; hydroxyebastine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Matsuda, M Dainippon Pharmaceut Co Ltd, Dev Res Labs, Dept Pharmacokinet, 33-94 EnokiCho, Osaka 5640053, Japan Dainippon Pharmaceut Co Ltd 33-94 Enoki Cho Osaka Japan 5640053
Citazione:
M. Matsuda et al., "Simultaneous determination of the histamine H-1-receptor antagonist ebastine and its two metabolites, carebastine and hydroxyebastine, in human plasma using high-performance liquid chromatography", J CHROMAT B, 757(1), 2001, pp. 173-179

Abstract

Ebastine (CAS 90729-43-4) is an antiallergic agent which selectively and potently blocks histamine H-1-receptors in vivo. A simple and sensitive high-performance liquid chromatography (HPLC) method is described for the simultaneous determination of ebastine and its two oxidized metabolites, carebastine (CAS 90729-42-3) and hydroxyebastine (M-OH), in human plasma. After a pretreatment of plasma sample by solid-phase extraction, ebastine and its metabolites were analyzed on an HPLC system with ultraviolet detection at 254 nm. Chromatography was performed on a cyano column (250x4.0 mm I.D.) at 40 degreesC with the mobile phase of acetonitrile-methanol-0.012 M ammonium acetate buffer (20.30:48, v/v/v) at a flow rate of 1.2 ml/min. Accurate determinations were possible over the concentration range of 3-1000 ng/ml for the three compounds using 1 ml plasma samples. The intra, and inter-day assay accuracy of this method were within 100+/-15% of nominal values and the precision did not exceed 12.4% of relative standard deviation. The lower limits of quantitation were 3 ng/ml for ebastine and its metabolites in humanplasma. This method was satisfactorily applied to the determination of ebastine and its two oxidized metabolites in human plasma after oral administration of ebastine. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 01:09:03