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Titolo:
Ultrasensitive assay for three polyphenols (catechin, quercetin and resveratrol) and their conjugates in biological fluids utilizing gas chromatography with mass selective detection
Autore:
Soleas, GJ; Yan, J; Goldberg, DM;
Indirizzi:
Univ Toronto, Banting Inst, Dept Lab Med & Pathobiol, Toronto, ON M5G 1L5,Canada Univ Toronto Toronto ON Canada M5G 1L5 hobiol, Toronto, ON M5G 1L5,Canada Liquor Control Board Ontario, Toronto, ON M5E 1A4, Canada Liquor Control Board Ontario Toronto ON Canada M5E 1A4 ON M5E 1A4, Canada
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 757, anno: 2001,
pagine: 161 - 172
SICI:
1387-2273(20010605)757:1<161:UAFTP
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; MODERATE ALCOHOL-CONSUMPTION; CORONARY HEART-DISEASE; HUMAN PLASMA; TRANS-RESVERATROL; RED-WINE; MYOCARDIAL-INFARCTION; URINARY-EXCRETION; SMALL-INTESTINE; BIOAVAILABILITY;
Keywords:
polyphenols; catechin; quercetin; resveratrol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Goldberg, DM Univ Toronto, Banting Inst, Dept Lab Med & Pathobiol, 100 Coll St, Toronto, ON M5G 1L5, Canada Univ Toronto 100 Coll St Toronto ON Canada M5G 1L5 L5, Canada
Citazione:
G.J. Soleas et al., "Ultrasensitive assay for three polyphenols (catechin, quercetin and resveratrol) and their conjugates in biological fluids utilizing gas chromatography with mass selective detection", J CHROMAT B, 757(1), 2001, pp. 161-172

Abstract

The concentrations of three polyphenols ((+)-catechin, quercetin and trans-resveratrol) in blood serum, plasma and urine, as well as whole blood, have been measured after their oral and intragastric administration, respectively, to humans and rats. The method developed for this purpose utilized ethyl acetate extraction of 100 mul samples and their derivatization with bis(trimethylsilyl)trifluoroacetamide (BSTFA) followed by gas-chromatographic analysis on a DB-5 column followed by mass selective detection employing twotarget ions and one qualifier ion for each compound. Total run time was 17min with excellent resolution and linearity. The limits of detection (LOD)and quantitation (LOQ) were an order of magnitude less than for any previously published method, being 0.01 mug/l and 0.1 mug/l, respectively, for all compounds. Recovery at 1 mug/l and 10 mug/l was >80% in all instances butone, and was >90% in 50%. Imprecision was acceptable at 0.25 and 1.0 mug/l, concentrations below the LOQ of previous methods. Aglycones released fromconjugates after hydrolysis were easily measurable. Optimal conditions forhydrolysis were established. After oral administration of the three polyphenols to humans, their conjugates vastly exceeded the concentrations of theaglycones in both plasma and urine. Concentrations peaked within 0.5-1.0 hin plasma and within 8 h in urine. During the first 24 h, 5.1% of the (+)-catechin and 24.6% of the trans-resveratrol given were recovered in the urine (free plus conjugated). This method can be proposed as the method of choice to assay these polyphenols and their conjugates in biological fluids. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 25/01/20 alle ore 09:52:39