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Titolo:
Determination of free D-aspartic acid, D-serine and D-alanine in the brainof mutant mice lacking D-amino-acid oxidase activity
Autore:
Morikawa, A; Hamase, K; Inoue, T; Konno, R; Niwa, A; Zaitsu, K;
Indirizzi:
Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ci, Higashi Ku, Fukuoka 8128582, Japan Dokkyo Univ, Sch Med, Dept Microbiol, Mibu, Tochigi 3210293, Japan Dokkyo Univ Mibu Tochigi Japan 3210293 biol, Mibu, Tochigi 3210293, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 757, anno: 2001,
pagine: 119 - 125
SICI:
1387-2273(20010605)757:1<119:DOFDAD>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; RAT-BRAIN; O-PHTHALDIALDEHYDE; DERIVATIZATION; PERIPHERY; RACEMASE; ORIGIN; SERUM;
Keywords:
enantiomer separation; D-amino-acid oxidase activity; aspartic acid; serine; alanine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Zaitsu, K Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 8128582, Japan
Citazione:
A. Morikawa et al., "Determination of free D-aspartic acid, D-serine and D-alanine in the brainof mutant mice lacking D-amino-acid oxidase activity", J CHROMAT B, 757(1), 2001, pp. 119-125

Abstract

A simple and precise method for the simultaneous determination of free D-aspartic acid, D-serine and D-alanine in mouse brain tissues was established, using a reversed-phase HPLC system with widely used pre-column derivatizing reagents, o-phthaldialdehyde and N-t-butyloxycarbonyl-L-cysteine. With the present method, the contents of these three D-amino acids in hippocampus. hypothalamus, pituitary gland, pineal gland and medulla oblongata as wellas cerebrum and cerebellum of mutant mice lacking D-amino-acid oxidase activity were determined and compared with those obtained for control mice. Inboth mice, extremely high contents of D-serine were observed in forebrain (100-400 nmol/g wet tissue), and the contents were small in pituitary and pineal glands. While, D-serine contents in cerebellum and medulla oblongata of mutant mice were about ten times higher than those in control mice. In contrast, D-alanine contents in mutant mice were higher than those in control mice in all brain regions and serum. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:09:07