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Titolo:
Simultaneous quantification of retinol, retinal, and retinoic acid isomersby high-performance liquid chromatography with a simple gradiation
Autore:
Miyagi, M; Yokoyama, W; Shiraishi, H; Matsumoto, M; Ishii, H;
Indirizzi:
Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 nal Med, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 757, anno: 2001,
pagine: 365 - 368
SICI:
1387-2273(20010615)757:2<365:SQORRA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE PROMYELOCYTIC LEUKEMIA;
Keywords:
vitamins; retinol; retinal; retinoic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoyama, W Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, 35 Shinanomachi, Tokyo1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 o1608582, Japan
Citazione:
M. Miyagi et al., "Simultaneous quantification of retinol, retinal, and retinoic acid isomersby high-performance liquid chromatography with a simple gradiation", J CHROMAT B, 757(2), 2001, pp. 365-368

Abstract

A new method of high-performance liquid chromatography (HPLC) analysis to quantify isomers of retinol, retinal and retinoic acid simultaneously was established. The HPLC system consisted of a silica gel absorption column anda linear gradient with two kinds of solvents containing n-Hexane, 2-propanol, and glacial acetic acid in different ratios. It separated six retinoic acid isomers (13-cis, 9-cis, all-trans, all-trans-4-oxo, 9-cis-4-oxo, 13-cis-4-oxo), three retinal isomers (13-cis-, 9-cis-, and all-trans) and two retinol isomers (13-cis- and all-trans). Human serum samples were subjected to this HPLC analysis and at least, all-trans retinol, 13-cis retinol, and all-trans retinoic acid were detectable. This HPLC system is useful for evaluating retinoic acid formation from retinol via a two-step oxidation pathway. Moreover, it could he applied to monitoring the concentrations of various retinoids, including all-tuans retinoic acid in human sera. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:00:10