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Titolo:
Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography
Autore:
Sarasa-Nacenta, M; Lopez-Pua, Y; Mallolas, J; Blanco, JL; Gatell, JM; Carne, X;
Indirizzi:
Hosp Clin, Clin Pharmacol Unit, Barcelona, Spain Hosp Clin Barcelona Spain p Clin, Clin Pharmacol Unit, Barcelona, Spain Inst Invest Biomed August Pi & Sunyer, Infect Dis Serv, Hosp Clin, Barcelona 08036, Spain Inst Invest Biomed August Pi & Sunyer Barcelona Spain 08036 08036, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 757, anno: 2001,
pagine: 325 - 332
SICI:
1387-2273(20010615)757:2<325:SDOTHI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION; RAPID QUANTIFICATION;
Keywords:
indinavir; amprenavir; ritonavir; saquinavir; nelfinavir;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Sarasa-Nacenta, M Hosp Clin, Clin Pharmacol Unit, Barcelona, Spain Hosp Clin Barcelona Spain acol Unit, Barcelona, Spain
Citazione:
M. Sarasa-Nacenta et al., "Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high-performance liquid chromatography", J CHROMAT B, 757(2), 2001, pp. 325-332

Abstract

A rapid, simple and sensitive high-performance liquid chromatographic (HPLC) assay has been developed for the simultaneous quantification of tho HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma. The method involved the solid-phase extraction of thefive drugs and the internal standard (I.S., verapamil) from 400 mul of human plasma. The HPLC analysis used a reversed-phase C-18 analytical column and a mobile phase consisting of a gradient with 15 mM phosphate buffer (pH 5.75)-acetonitrile and UV monitoring. The method was linear over the therapeutic concentration range for the five HIV-protease inhibitors. The accuracy of the method ranged from 98.2 to 106.7%, and the precision values rangedfrom 1.4 to 8.1% for intra-day precision and from 3.1 to 6.4% for the inter-day values. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:30:33