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Titolo:
Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis
Autore:
Stenderup, K; Justesen, J; Eriksen, EF; Rattan, SIS; Kassem, M;
Indirizzi:
Univ Aarhus, Aarhus Kommune Hosp, Dept Endocrinol & Metab, DK-8000 Aarhus C, Denmark Univ Aarhus Aarhus Denmark C docrinol & Metab, DK-8000 Aarhus C, Denmark Univ Aarhus, Inst Mol & Struct Biol, Lab Cellular Aging, DK-8000 Aarhus, Denmark Univ Aarhus Aarhus Denmark DK-8000 llular Aging, DK-8000 Aarhus, Denmark
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 6, volume: 16, anno: 2001,
pagine: 1120 - 1129
SICI:
0884-0431(200106)16:6<1120:NAPCOO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BONE-MARROW; OSTEOBLAST-LIKE CELLS; GROWTH-FACTOR-SYSTEM; ADULT HUMAN BONE; POSTMENOPAUSAL OSTEOPOROSIS; IN-VITRO; STROMAL CELLS; PRECURSOR CELLS; AGED RATS; INVITRO;
Keywords:
human bone marrow; mesenchymal stem cells; colony-forming unit-fibroblastic; aging; STRO-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kassem, M Univ Aarhus, Aarhus Kommune Hosp, Dept Endocrinol & Metab, DK-8000 Aarhus C, Denmark Univ Aarhus Aarhus Denmark C Metab, DK-8000 Aarhus C, Denmark
Citazione:
K. Stenderup et al., "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis", J BONE MIN, 16(6), 2001, pp. 1120-1129

Abstract

Decreased bone formation is an important pathophysiological mechanism responsible for bone loss associated with aging and osteoporosis. Osteoblasts (OBs), originate from mesenchymal stem cells (MSCs) that are present in the bone marrow and form colonies (termed colony-forming units-fibroblastic [CFU-Fs]) when cultured in vitro. To examine the effect of aging and osteoporosis on the MSC population, we quantified the number of MSCs and their proliferative capacity in vitro. Fifty-one individuals were studied: 38 normal volunteers (23 young individuals [age, 22-44 years] and 15 old individuals [age, 66-74 years]) and 13 patients with osteoporosis (age, 58-83 years). Bone marrow was aspirated from iliac crest; mononuclear cells were enriched in MSCs by magnetic activated cell sorting (MACS) using STRO-1 antibody. Total CFU-F number, size distribution, cell density per CFU-F, number of alkaline phosphatase positive (ALP(+)) CFU-Fs, and the total ALP(+) cells were determined. In addition, matrix mineralization as Estimated by alizarin red S (AR-S) staining was quantified. No significant difference in colony-forming efficiency between young individuals (mean a SEM; 87 +/- 12 CFU-Fs/culture), old individuals (99 +/- 19 CFU-Fs/culture), and patients with osteoporosis (129 +/- 13 CFU-Fs/culture; p = 0.20) was found. Average CFU-F size and cell density per colony were similar in the three groups. Neither the percentage of ALP(+) CFU-Fs (66 +/- 6%, 65 +/- 7%, and 72 +/- 4% for young individuals, old individuals, and patients with osteoporosis, respectively) nor the percentage of ALP(+) cells per culture (34 +/- 5%, 40 +/- 6%, and 41 /- 4%) differed between groups. Finally, mineralized matrix formation was similar in young individuals, old individuals, and patients with osteoporosis. Our study shows that the number and proliferative capacity of osteoprogenitor cells are maintained during aging and in patients with osteoporosis and that other mechanisms must be responsible for the defective osteoblast (OB) functions observed in these conditions.

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Documento generato il 09/04/20 alle ore 19:25:31