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Titolo:
Ca2+-images of smooth muscle cells and endothelial cells in one confocal plane in femoral artery segments of the rat
Autore:
Ohi, Y; Takai, N; Muraki, K; Watanabe, M; Imaizumi, Y;
Indirizzi:
Nagoya City Univ, Grad Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Nagoya, Aichi 4678603, Japan Nagoya City Univ Nagoya Aichi Japan 4678603 Nagoya, Aichi 4678603, Japan
Titolo Testata:
JAPANESE JOURNAL OF PHARMACOLOGY
fascicolo: 1, volume: 86, anno: 2001,
pagine: 106 - 113
SICI:
0021-5198(200105)86:1<106:COSMCA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; DEPENDENT HYPERPOLARIZATION; CA2+;
Keywords:
Ca2+-imaging; rat femoral artery; endothelium; confocal microscope;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Imaizumi, Y Nagoya City Univ, Grad Sch Pharmaceut Sci, Dept Mol & CellularPharmacol, Nagoya, Aichi 4678603, Japan Nagoya City Univ Nagoya Aichi Japan 4678603 hi 4678603, Japan
Citazione:
Y. Ohi et al., "Ca2+-images of smooth muscle cells and endothelial cells in one confocal plane in femoral artery segments of the rat", JPN J PHARM, 86(1), 2001, pp. 106-113

Abstract

Simultaneous recording of Ca2+-images in one confocal plane from vascular smooth muscle cells (SMCs) and endothelial cells (ECs) of an intact rat femoral artery segment was performed using indo-1 and a confocal microscope. During application of 10 muM acetylcholine (ACh), elevation and oscillation of intracellular Ca2+ concentration ([Ca2+](i)) were observed in ECs but not in SMCs. Sequential conduction of Ca2+ oscillation from an EC to the neighboring ECs in one longitudinal direction was often observed in the presence of ACh. On the other hand, the activation of voltage-dependent Ca2+ channels by external 30 mM K+ resulted in the elevation of [Ca2+](i) only in SMCs. When 10 muM ACh was added in the presence of 30 mM K+, it was observed in one confocal plane that [Ca2+](i) in ECs and SMCs was almost simultaneously increased and decreased, respectively. The simultaneous recording methodin this intact preparation will provide a line of valuable information about the interactions between SMCs and ECs, based on spatio-temporal analysesof absolute values of [Ca2+](i) in individual cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:07:48