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Titolo:
Interaction between monensin and CCl4 after chronic treatment: Dolichol and retinol content of rat liver sinusoidal cells
Autore:
Nanni, G; Majorani, F; Maloberti, G; Canepa, C; Casu, A;
Indirizzi:
Univ Genoa, Dept Expt Med, Gen Pathol Sect, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 ed, Gen Pathol Sect, I-16132 Genoa, Italy
Titolo Testata:
INTERNATIONAL JOURNAL OF TISSUE REACTIONS-EXPERIMENTAL AND CLINICAL ASPECTS
fascicolo: 1, volume: 23, anno: 2001,
pagine: 9 - 20
SICI:
0250-0868(2001)23:1<9:IBMACA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATIC STELLATE CELLS; FAT-STORING CELLS; MULTIDRUG TRANSPORTER; BINDING PROTEINS; P-GLYCOPROTEIN; PARENCHYMAL-CELLS; KUPFFER CELLS; VITAMIN-A; IN-VIVO; MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Casu, A Univ Genoa, Dept Expt Med, Gen Pathol Sect, Via LB Alberti 2, I-16132 Genoa, Italy Univ Genoa Via LB Alberti 2 Genoa Italy I-16132 6132 Genoa, Italy
Citazione:
G. Nanni et al., "Interaction between monensin and CCl4 after chronic treatment: Dolichol and retinol content of rat liver sinusoidal cells", INT J TISS, 23(1), 2001, pp. 9-20

Abstract

The aim of this investigation was to study retinol, which is known to decrease in hepatic stellate cells during fibrogenesis, and dolichol, which influences membrane fluidity and decreases in liver injury, in freshly isolated liver parenchymal and nonparenchymal cells after intoxication of rats with CCl4 combined with the ionophore monensin for 3, 5 and 7 weeks. To study the interrelationship between dolichol and vitamin A transport, a load of vitamin A was given to batches of rats 3 days before sacrifice. Monensin didnot modify the action of CCl4 in hepatocytes. On administration of CCl4 and CCl4-monensin, dolichol decreased independently of vitamin A load, while retinol increased, especially when a load of vitamin A was given to rats 3 days before sacrifice. Hepatocytes appeared to no longer be able to export or metabolize vitamin A. In a subfraction of hepatic stellate cells (Ito-l cells) dolichol always decreased, while retinol was no longer stored after each trealment; dolichol and retinol showed the same behavior but the decrease was more pronounced in monensin after vitamin A load and after 3 weeks. These data support the hypothesis that by modulating membrane characteristics, dolichol might be involved in intracellular or intercellular retinol transport and that altered transport between hepatocytes and Ito-1 cells might accompany liver injury: The data regarding another subfraction, Ito-2 cells, partly resemble those for the Ito-1 fraction and are in agreement withthe heterogeneity of hepatic stellate cells. In Kupffer and sinusoidal endothelial cells, dolichol and retinol content was not homogeneous and was only slightly altered after the treatments. Monensin and CC!, are not interactive. Although both drugs alter membrane lipids, their association allows some sinusoidal cell responses to be differentiated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:13:38