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Titolo:
Sphingomonas pituitosa sp nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan
Autore:
Denner, EBM; Paukner, S; Kampfer, P; Moore, ERB; Abraham, WR; Busse, HJ; Wanner, G; Lubitz, W;
Indirizzi:
Univ Vienna, Inst Mikrobiol & Genet, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 robiol & Genet, A-1030 Vienna, Austria Univ Giessen, Inst Angew Mikrobiol, D-35390 Giessen, Germany Univ GiessenGiessen Germany D-35390 Mikrobiol, D-35390 Giessen, Germany Gesell Biotechnol Forsch, Bereich Mikrobiol, D-38124 Braunschweig, GermanyGesell Biotechnol Forsch Braunschweig Germany D-38124 unschweig, Germany Univ Vienna, Inst Bakteriol Mykol & Hyg Vet Med, A-1210 Vienna, Austria Univ Vienna Vienna Austria A-1210 & Hyg Vet Med, A-1210 Vienna, Austria Univ Munich, Inst Bot, D-80683 Munich, Germany Univ Munich Munich Germany D-80683 ch, Inst Bot, D-80683 Munich, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
, volume: 51, anno: 2001,
parte:, 3
pagine: 827 - 841
SICI:
1466-5026(200105)51:<827:SPSNAE>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; RIBOSOMAL-DATABASE-PROJECT; GENUS SPHINGOMONAS; EXTRACELLULAR POLYSACCHARIDE; COMB-NOV; PSEUDOMONAS-PAUCIMOBILIS; RENATURATION RATES; AROMATIC-COMPOUNDS; DNA HYBRIDIZATION; URSINCOLA YURKOV;
Keywords:
Sphingomonas pituitosa sp nov.; polyphasic characterization; exopolysaccharide; sphingan;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
104
Recensione:
Indirizzi per estratti:
Indirizzo: Denner, EBM Univ Vienna, Inst Mikrobiol & Genet, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 et, A-1030 Vienna, Austria
Citazione:
E.B.M. Denner et al., "Sphingomonas pituitosa sp nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan", INT J SY EV, 51, 2001, pp. 827-841

Abstract

Strain EDIVT, an exopolysaccharide-producing bacterium, was subjected to polyphasic characterization. The bacterium produced copious amounts of an extracellular polysaccharide, forming slimy, viscous, intensely yellow-pigmented colonies on Czapek-Dox (CZD) agar. The culture fluids of the liquid version of CZD medium were highly viscous after cultivation for 5 d. Cells of strain EDIVT were Gram-negative, catalase-positive, oxidase-negative, nonspore-forming, rod-shaped and motile. Comparisons of 16S rDNA gene sequences demonstrated that EDIVT clusters phylogenetically with the species of the genus Sphingomonas sensu stricto. The G+C content of the DNA (64.5 mol%), the presence of ubiquinone Q-10, the presence of 2-hydroxymyristic acid (14:02-OH) as the major hydroxylated fatty acid, the absence of 3-hydroxy fattyacids and the detection of sym-homospermidine as the major component in the polyamine pattern, together with the presence of sphingoglycolipid, supported this delineation. 16S rDNA sequence analysis indicated that strain EDIVT is most closely related (99.4% similarity) to Sphingomonas trueperi LMG 2142(T). DNA-DNA hybridization showed that the level of relatedness to 5. trueperi is only 45.5%. Further differences were apparent in the cellular fatty acid profile, the polar lipid pattern, the Fourier-transform infrared spectrum and whole-cell proteins and in a number of biochemical characteristics. On the basis of the estimated phylogenetic position derived from 16S rDNA sequence data, DNA-DNA reassociation and phenotypic differences, strainEDIVT (= CIP 106154(T) = DSM 13101(T)) was recognized as a new species of Sphingomonas, for which the name Sphingomonas pituitosa sp. nov. is proposed. A component analysis of the exopolysaccharide (named PS-EDIV) suggested that it represents a novel type of sphingan composed of glucose, rhamnose and an unidentified sugar. Glucuronic acid, which is commonly found in sphingans, was absent. The mean molecular mass of PS-EDIV was approximately 3 x 10(6) Da.

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Documento generato il 05/12/20 alle ore 14:07:00