Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Clostridium uliginosum sp nov., a novel acid-tolerant anaerobic bacterium with connecting filaments
Autore:
Matthies, C; Kuhner, CH; Acker, G; Drake, HL;
Indirizzi:
Univ Bayreuth, BITOEK, Dept Ecol Microbiol, D-95440 Bayreuth, Germany UnivBayreuth Bayreuth Germany D-95440 robiol, D-95440 Bayreuth, Germany Univ Bayreuth, Electron Microscopy Lab, D-95440 Bayreuth, Germany Univ Bayreuth Bayreuth Germany D-95440 py Lab, D-95440 Bayreuth, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
, volume: 51, anno: 2001,
parte:, 3
pagine: 1119 - 1125
SICI:
1466-5026(200105)51:<1119:CUSNAN>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; FOREST SOILS; METHANE PRODUCTION; BEECH FOREST; THERMOACETICUM; DENITRIFICATION; PH; PEATLANDS; NITRATE; LITTER;
Keywords:
acidic forest soil; acid tolerance; Clostridium uliginosum; connecting filaments;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Drake, HL Univ Bayreuth, BITOEK, Dept Ecol Microbiol, POB 101251, D-95440 Bayreuth, Germany Univ Bayreuth POB 101251 Bayreuth Germany D-95440 euth, Germany
Citazione:
C. Matthies et al., "Clostridium uliginosum sp nov., a novel acid-tolerant anaerobic bacterium with connecting filaments", INT J SY EV, 51, 2001, pp. 1119-1125

Abstract

An anaerobic, acid-tolerant bacterium, CK55(T), was isolated from an acidic forest bog. Cells of CK55(T) stained Gram-negative but did not have an outer membrane. Cells were spore-forming, motile rods with peritrichous flagella, formed chains or aggregates and were linked by connecting filaments that were composed of a core and outer sheath. Cellobiose, glucose, xylose, mannose, mannitol, sucrose and peptone supported growth. Arabinose, lactose,raffinose, H-2/CO2, CO/CO2, vanillate, Casamino acids and various purines and pyrimidines did not support growth. Growth on carbohydrates yielded acetate, butyrate, lactate, formate and H, as end-products. Growth was observed at pH 4.0-9.0, with an optimum at pH 6.5, and at 10-30 degreesC, with an optimum at 20-25 degreesC. At 20 degreesC, doubling times were 4 and 6 h atpH 6.5 and 4.0, respectively. Hydrogenase activity in cell-free extracts was 12 U (mg protein)(-1) CK55(T) did not: (i) contain detectable levels of CO, formate, lactate dehydrogenases or cytochromes; (ii) carry out dissimilatory reduction of nitrate or sulfate; or (iii) produce methane. Thus, CK55T was characterized as a non-acetogenic, fermentative chemo-organotroph. The G+C content of CK55T was 28.0 mol%. CK55(T) was phylogenetically most closely related to Clostridium botulinum (types B, E and 6), Clostridium acetobutylicum and other saccharolytic clostridia; the 16S rRNA gene sequence similarity values to the nearest relatives of CK55T were approximately 97%. Based on morphological, physiological and phylogenetic properties of CK55T, it is proposed that CK55T be termed Clostridium uliginosum sp. nov. (= DSM 12992(T) = ATCC BAA-53(T).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:38:23