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Titolo:
Overexpression of Bcl-2 regulates sodium butyrate- and/or docetaxel-induced apoptosis in human bladder cancer cells both in vitro and in vivo
Autore:
Miyake, H; Hara, S; Arakawa, S; Kamidono, S; Hara, I;
Indirizzi:
Kobe Univ, Sch Med, Dept Urol, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 l, Chuo Ku, Kobe, Hyogo 6500017, Japan Vancouver Gen Hosp, Prostate Ctr, Vancouver, BC, Canada Vancouver Gen Hosp Vancouver BC Canada ostate Ctr, Vancouver, BC, Canada
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 1, volume: 93, anno: 2001,
pagine: 26 - 32
SICI:
0020-7136(20010701)93:1<26:OOBRSB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTATE-CANCER; P53 EXPRESSION; BREAST-CANCER; TUMOR-MODEL; CARCINOMA; CHEMOTHERAPY; RADIOTHERAPY; PROGRESSION; AGENTS; DEATH;
Keywords:
sodium butyrate; Bcl-2; docetaxel; bladder cancer; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Miyake, H Kobe Univ, Sch Med, Dept Urol, Chuo Ku, 7-5-1 Kusunoki Cho, Kobe, Hyogo 6500017, Japan Kobe Univ 7-5-1 Kusunoki Cho Kobe Hyogo Japan 6500017 017, Japan
Citazione:
H. Miyake et al., "Overexpression of Bcl-2 regulates sodium butyrate- and/or docetaxel-induced apoptosis in human bladder cancer cells both in vitro and in vivo", INT J CANC, 93(1), 2001, pp. 26-32

Abstract

Sodium butyrate (NaBt), a physiologically occurring short-chain fatty acid, induces differentiation as well as apoptosis in numerous cell types, and this induction is partially regulated by Bcl-2 expression. The objectives of our study were to characterize the in vitro effects of NaBt and/or docetaxel on the growth, cell cycle and apoptosis of human bladder cancer cells, and to determine whether tumor growth in vivo is inhibited by isobutyramide, an orally bioavailable Bt analogue, and/or docetaxel by using Bcl-2-transfected human bladder cancer cell line KoTCC-I/BH and control vector only-transfected cell line KoTCC-I/C. NaBt caused a decrease in growth of both KoTCC-I/C and KoTCC-I/BH cells, however, its growth inhibitory effect was significantly greater in KoTCC-I/C cells. One mM NaBt resulted in CI cell cyclearrest, accompanied by up-regulation of p21 (wafI/cipI) and down-regulation of cyclin DI in KoTCC-I/C cells, whereas KoTCC-I/BH showed resistance to GI cell cycle arrest. An amount of 5 mM NaBt induced apoptosis, accompaniedby up-regulation of Bak in KoTCC-I/C cells but failed to induce apoptosis in KoTCC-I/BH cells despite substantial down-regulation of Bcl-2. Oral administration of isobutyramide significantly reduced the KoTCC-I/C tumor volume compared with the KoTCC-I/BH tumor volume in nude mice. Furthermore, docetaxel induced Bcl-2 phosphorylation in KoTCC-I/BH cells and combined treatment with isobutyramide and docetaxel synergistically inhibited the growth of KoTCC-I/BH cells both in vitro and in vivo. These findings suggest that isobutyramide therapy could be a novel therapeutic strategy for patients with bladder cancer if docetaxel is combined according to the Bcl-2 expressionstatus. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:04:34